Detailed information of TA system TA096726

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3955822..3956516
• Replicon: chromosome
• Accession: NZ_CP026935
• Organism: Escherichia coli strain CFS3292

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A1X9TDN4
• Locus tag: C5F72_RS19485
• Protein ID: WP_001094398.1
• Coordinates: 3955822..3956190 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: C5F72_RS19490
• Protein ID: WP_077249034.1
• Coordinates: 3956211..3956516 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5F72_RS19465	3950995..3951951	+	957	WP_000643333.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-
C5F72_RS19470	3951948..3954146	+	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
C5F72_RS19475	3954156..3955112	+	957	WP_000121359.1	molybdenum cofactor insertion chaperone PaoD	-
C5F72_RS19480	3955163..3955501	+	339	Protein_3820	LysR family transcriptional regulator	-
C5F72_RS19485	3955822..3956190	-	369	WP_001094398.1	TA system toxin CbtA family protein	Toxin
C5F72_RS19490	3956211..3956516	-	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C5F72_RS19495	3956620..3957264	-	645	WP_021566097.1	hypothetical protein	-
C5F72_RS19500	3957283..3957504	-	222	WP_000692345.1	DUF987 domain-containing protein	-
C5F72_RS19505	3957573..3958049	-	477	WP_001424026.1	RadC family protein	-
C5F72_RS19510	3958065..3958538	-	474	WP_000855059.1	antirestriction protein	-
C5F72_RS19515	3958880..3959701	-	822	WP_021566099.1	DUF945 domain-containing protein	-
C5F72_RS19520	3959802..3960010	-	209	Protein_3828	DUF905 family protein	-
C5F72_RS19525	3960111..3960566	-	456	WP_000581506.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13581.73 Da
• Isoelectric Point: 7.7465

>T96726 WP_001094398.1 NZ_CP026935:c3956190-3955822 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
NSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T96726 NZ_CP026935:c3956190-3955822 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCTCCGGCGGCGAAGCCCTGTCCGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTTTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATTG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
AACTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGTTGATGAGCCGGTG
TTCTTACAGAGATGTAAGTAATATTGTTCTTAGCCGATCACGGTTGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT96726 WP_077249034.1 NZ_CP026935:c3956516-3956211 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT96726 NZ_CP026935:c3956516-3956211 [Escherichia coli]
CTGGTGGGGCTACCCTACACCGTGACGCCCTGTTCTGGCACCCGTCTGGTGCAGGAGGGCAACCGACTGTACTATTTGGC
TGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGCTGGATGTCGTATTTCCACATTTTATCAGTC
AGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTTTCACCCTGTACCACAACGGTTTCACC
TGCGAAGCCGATACCCTTGGTAGTTGCGGCTATGTATACATCGCCATTTATCCCACTCAGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TDN4