Detailed information of TA system TA096722

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2504827..2505353
• Replicon: chromosome
• Accession: NZ_CP026935
• Organism: Escherichia coli strain CFS3292

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: C5F72_RS12185
• Protein ID: WP_000323025.1
• Coordinates: 2505066..2505353 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: C5F72_RS12180
• Protein ID: WP_000534858.1
• Coordinates: 2504827..2505066 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5F72_RS12155	2499941..2500192	+	252	Protein_2381	DUF977 family protein	-
C5F72_RS12160	2500189..2500545	+	357	WP_001310834.1	class I SAM-dependent methyltransferase	-
C5F72_RS27435	2501156..2501443	+	288	Protein_2383	hypothetical protein	-
C5F72_RS12165	2502212..2503525	+	1314	WP_000589005.1	ISNCY family transposase	-
C5F72_RS12170	2503962..2504294	-	333	WP_001301033.1	protein FlxA	-
C5F72_RS12175	2504497..2504802	-	306	WP_001326990.1	hypothetical protein	-
C5F72_RS12180	2504827..2505066	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
C5F72_RS12185	2505066..2505353	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
C5F72_RS12190	2505425..2505580	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
C5F72_RS12195	2505797..2506048	+	252	WP_000980994.1	hypothetical protein	-
C5F72_RS12200	2506115..2506393	+	279	WP_012304870.1	hypothetical protein	-
C5F72_RS12205	2506395..2507444	+	1050	WP_001265198.1	DUF968 domain-containing protein	-
C5F72_RS12210	2507458..2508210	+	753	WP_061093371.1	antitermination protein	-
C5F72_RS12215	2508488..2508577	-	90	WP_120795389.1	hypothetical protein	-
C5F72_RS12220	2508632..2508844	-	213	WP_000087756.1	cold shock-like protein CspF	-
C5F72_RS12225	2509145..2509360	+	216	WP_000066484.1	cold shock-like protein CspB	-
C5F72_RS12230	2510114..2510329	+	216	WP_000839590.1	phage lysis protein EssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2496330..2521077	24747

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T96722 WP_000323025.1 NZ_CP026935:2505066-2505353 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T96722 NZ_CP026935:2505066-2505353 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT96722 WP_000534858.1 NZ_CP026935:2504827-2505066 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT96722 NZ_CP026935:2504827-2505066 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8