Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 120344..120869 Replicon plasmid pCFS3273-2
Accession NZ_CP026934
Organism Escherichia coli strain CFS3273

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag C5F73_RS26635 Protein ID WP_001159871.1
Coordinates 120344..120649 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag C5F73_RS26640 Protein ID WP_000813630.1
Coordinates 120651..120869 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5F73_RS26620 116306..117472 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
C5F73_RS26625 118060..118815 - 756 WP_000852146.1 replication initiation protein RepE -
C5F73_RS26630 119537..120343 - 807 WP_000016970.1 site-specific integrase -
C5F73_RS26635 120344..120649 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
C5F73_RS26640 120651..120869 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C5F73_RS26645 121497..121727 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
C5F73_RS26650 121724..122140 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
C5F73_RS26655 122215..123780 + 1566 WP_001128474.1 AAA family ATPase -
C5F73_RS26660 123765..124787 + 1023 WP_000361402.1 helicase UvrD -
C5F73_RS26665 125041..125727 - 687 Protein_150 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / tet(B) / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / sul2 / sitABCD iutA / iucD / iucC / iucB / iucA 1..145001 145001


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T96699 WP_001159871.1 NZ_CP026934:c120649-120344 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T96699 NZ_CP026934:c120649-120344 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT96699 WP_000813630.1 NZ_CP026934:c120869-120651 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT96699 NZ_CP026934:c120869-120651 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References