Detailed information of TA system TA096689

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 56828..57375
• Replicon: plasmid pCFS3273-2
• Accession: NZ_CP026934
• Organism: Escherichia coli strain CFS3273

Toxin (Protein)

• Gene name: parE
• Uniprot ID: E9LLH3
• Locus tag: C5F73_RS26250
• Protein ID: WP_000969996.1
• Coordinates: 56828..57109 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: K4JYR0
• Locus tag: C5F73_RS26255
• Protein ID: WP_000079928.1
• Coordinates: 57106..57375 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5F73_RS26215	52340..53155	-	816	WP_001043260.1	sulfonamide-resistant dihydropteroate synthase Sul2	-
C5F73_RS26220	53370..53642	+	273	WP_000019163.1	hypothetical protein	-
C5F73_RS26225	53623..54831	+	1209	WP_000198533.1	IS91 family transposase	-
C5F73_RS26230	55003..55182	-	180	Protein_63	hypothetical protein	-
C5F73_RS26235	55534..55788	+	255	WP_000343103.1	hypothetical protein	-
C5F73_RS26240	55788..56378	+	591	WP_000194550.1	hypothetical protein	-
C5F73_RS26245	56396..56743	-	348	WP_001115718.1	hypothetical protein	-
C5F73_RS26250	56828..57109	-	282	WP_000969996.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C5F73_RS26255	57106..57375	-	270	WP_000079928.1	hypothetical protein	Antitoxin
C5F73_RS26260	58289..59146	-	858	WP_000131010.1	incFII family plasmid replication initiator RepA	-
C5F73_RS26265	59139..59213	-	75	WP_001365705.1	RepA leader peptide Tap	-
C5F73_RS26270	59460..59708	-	249	WP_000083835.1	replication regulatory protein RepA	-
C5F73_RS26275	59992..60198	-	207	WP_001553767.1	type I toxin-antitoxin system Hok family toxin	-
-	60185..60246	+	62	NuclAT_2	-	-
-	60185..60246	+	62	NuclAT_2	-	-
-	60185..60246	+	62	NuclAT_2	-	-
-	60185..60246	+	62	NuclAT_2	-	-
C5F73_RS26280	60421..60803	-	383	Protein_73	hypothetical protein	-
C5F73_RS26285	60906..61367	-	462	WP_000760078.1	thermonuclease family protein	-
C5F73_RS26290	62110..62313	-	204	WP_001327131.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / tet(B) / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / sul2 / sitABCD	iutA / iucD / iucC / iucB / iucA	1..145001	145001

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10893.57 Da
• Isoelectric Point: 8.4615

>T96689 WP_000969996.1 NZ_CP026934:c57109-56828 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T96689 NZ_CP026934:c57109-56828 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCACATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGAGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10175.50 Da
• Isoelectric Point: 7.3210

>AT96689 WP_000079928.1 NZ_CP026934:c57375-57106 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT96689 NZ_CP026934:c57375-57106 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGATCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGCGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCATCTGCTGCAGCATGGAGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: E9LLH3

Antitoxin

• AlphaFold DB: A0A5W2J4D0