Detailed information of TA system TA096685

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4621255..4622053
• Replicon: chromosome
• Accession: NZ_CP026932
• Organism: Escherichia coli strain CFS3273

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1X1M0U2
• Locus tag: C5F73_RS22150
• Protein ID: WP_000854919.1
• Coordinates: 4621676..4622053 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: C5F73_RS22145
• Protein ID: WP_159373447.1
• Coordinates: 4621255..4621629 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5F73_RS22110	4616920..4617600	+	681	WP_001282921.1	WYL domain-containing protein	-
C5F73_RS22115	4617748..4618425	+	678	WP_001097302.1	hypothetical protein	-
C5F73_RS22120	4618431..4618664	+	234	WP_001278293.1	DUF905 family protein	-
C5F73_RS22125	4618754..4619572	+	819	WP_001175165.1	DUF945 domain-containing protein	-
C5F73_RS22130	4619838..4620317	+	480	WP_000706978.1	antirestriction protein	-
C5F73_RS22135	4620332..4620808	+	477	WP_159373446.1	RadC family protein	-
C5F73_RS22140	4620871..4621092	+	222	WP_000692329.1	DUF987 domain-containing protein	-
C5F73_RS22145	4621255..4621629	+	375	WP_159373447.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C5F73_RS22150	4621676..4622053	+	378	WP_000854919.1	TA system toxin CbtA family protein	Toxin
C5F73_RS22155	4622050..4622538	+	489	WP_159373448.1	hypothetical protein	-
C5F73_RS22160	4622550..4622747	+	198	WP_000839281.1	DUF957 domain-containing protein	-
C5F73_RS22165	4622844..4623413	+	570	WP_001290247.1	DUF4942 domain-containing protein	-
C5F73_RS22170	4623787..4624767	+	981	WP_000019440.1	IS5-like element ISKpn26 family transposase	-
C5F73_RS22175	4625361..4626899	+	1539	WP_001187173.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4608376..4634711	26335
	-	flank	IS/Tn	-	-	4623787..4624767	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14037.97 Da
• Isoelectric Point: 7.9086

>T96685 WP_000854919.1 NZ_CP026932:4621676-4622053 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGTSSQLINSIDILRARRATGLMTRSNYRTVNNITLGKHPEAKQ

Nucleotide

• Length: 378 bp

>T96685 NZ_CP026932:4621676-4622053 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCAGGAACTTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13577.44 Da
• Isoelectric Point: 5.9445

>AT96685 WP_159373447.1 NZ_CP026932:4621255-4621629 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPHRQNTVTLYAKGLTCHADTLGSCGYVYLAVYPTPAPAITS

Nucleotide

• Length: 375 bp

>AT96685 NZ_CP026932:4621255-4621629 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCACCGTCAGAATACCGTCACGCTGTATGCTAAAGGGCTGACCTGCCATGCGGACACCCTCGGCAGCTG
TGGTTACGTTTATCTTGCTGTTTATCCGACACCGGCACCCGCAATCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A1X1M0U2