Detailed information of TA system TA096680

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3489512..3490344
• Replicon: chromosome
• Accession: NZ_CP026932
• Organism: Escherichia coli strain CFS3273

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: C5F73_RS16940
• Protein ID: WP_000854765.1
• Coordinates: 3489970..3490344 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: C5F73_RS16935
• Protein ID: WP_001295723.1
• Coordinates: 3489512..3489880 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5F73_RS16910	3486627..3486785	+	159	WP_061349359.1	DUF905 family protein	-
C5F73_RS16915	3486940..3487758	+	819	WP_001234729.1	DUF945 domain-containing protein	-
C5F73_RS16920	3488100..3488573	+	474	WP_000855059.1	antirestriction protein	-
C5F73_RS16925	3488589..3489065	+	477	WP_001186773.1	RadC family protein	-
C5F73_RS16930	3489128..3489349	+	222	WP_001556363.1	DUF987 domain-containing protein	-
C5F73_RS16935	3489512..3489880	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C5F73_RS16940	3489970..3490344	+	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
C5F73_RS16945	3490341..3490547	+	207	WP_122997564.1	hypothetical protein	-
C5F73_RS26840	3490560..3490673	+	114	WP_001161660.1	DUF957 domain-containing protein	-
C5F73_RS16950	3490832..3491860	+	1029	Protein_3319	IS110 family transposase	-
C5F73_RS16955	3492402..3492806	+	405	WP_000839179.1	IS66 family insertion sequence hypothetical protein	-
C5F73_RS16960	3492803..3493150	+	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
C5F73_RS16965	3493199..3494734	+	1536	WP_001333339.1	IS66-like element ISEc22 family transposase	-
C5F73_RS16970	3494777..3494959	+	183	WP_024167529.1	ethanolamine utilization protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T96680 WP_000854765.1 NZ_CP026932:3489970-3490344 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T96680 NZ_CP026932:3489970-3490344 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGACTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT96680 WP_001295723.1 NZ_CP026932:3489512-3489880 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT96680 NZ_CP026932:3489512-3489880 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52