Detailed information of TA system TA096669

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 1675095..1675789
• Replicon: chromosome
• Accession: NZ_CP026932
• Organism: Escherichia coli strain CFS3273

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A1X9TDN4
• Locus tag: C5F73_RS08115
• Protein ID: WP_001094398.1
• Coordinates: 1675421..1675789 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: C5F73_RS08110
• Protein ID: WP_077249034.1
• Coordinates: 1675095..1675400 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5F73_RS08075	1671045..1671500	+	456	WP_000581506.1	hypothetical protein	-
C5F73_RS08080	1671601..1671809	+	209	Protein_1584	DUF905 family protein	-
C5F73_RS08085	1671910..1672731	+	822	WP_001234565.1	DUF945 domain-containing protein	-
C5F73_RS08090	1673073..1673546	+	474	WP_000855059.1	antirestriction protein	-
C5F73_RS08095	1673562..1674038	+	477	WP_001424026.1	RadC family protein	-
C5F73_RS08100	1674107..1674328	+	222	WP_000692345.1	DUF987 domain-containing protein	-
C5F73_RS08105	1674347..1674991	+	645	WP_000086755.1	hypothetical protein	-
C5F73_RS08110	1675095..1675400	+	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C5F73_RS08115	1675421..1675789	+	369	WP_001094398.1	TA system toxin CbtA family protein	Toxin
C5F73_RS08120	1676110..1676520	-	411	WP_001111348.1	hypothetical protein	-
C5F73_RS08125	1676499..1677455	-	957	WP_000121359.1	molybdenum cofactor insertion chaperone PaoD	-
C5F73_RS08130	1677465..1679663	-	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
C5F73_RS08135	1679660..1680616	-	957	WP_000643333.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1660152..1682334	22182

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13581.73 Da
• Isoelectric Point: 7.7465

>T96669 WP_001094398.1 NZ_CP026932:1675421-1675789 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
NSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T96669 NZ_CP026932:1675421-1675789 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCTCCGGCGGCGAAGCCCTGTCCGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTTTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATTG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
AACTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGTTGATGAGCCGGTG
TTCTTACAGAGATGTAAGTAATATTGTTCTTAGCCGATCACGGTTGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT96669 WP_077249034.1 NZ_CP026932:1675095-1675400 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT96669 NZ_CP026932:1675095-1675400 [Escherichia coli]
CTGGTGGGGCTACCCTACACCGTGACGCCCTGTTCTGGCACCCGTCTGGTGCAGGAGGGCAACCGACTGTACTATTTGGC
TGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGCTGGATGTCGTATTTCCACATTTTATCAGTC
AGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTTTCACCCTGTACCACAACGGTTTCACC
TGCGAAGCCGATACCCTTGGTAGTTGCGGCTATGTATACATCGCCATTTATCCCACTCAGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TDN4