Detailed information of TA system TA096659

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 96458..97292
• Replicon: chromosome
• Accession: NZ_CP026932
• Organism: Escherichia coli strain CFS3273

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: C5F73_RS00485
• Protein ID: WP_001518317.1
• Coordinates: 96458..96835 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: C5F73_RS00490
• Protein ID: WP_024187829.1
• Coordinates: 96924..97292 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5F73_RS00455	91658..92842	-	1185	WP_001172898.1	sugar efflux transporter	-
C5F73_RS00460	93224..93370	-	147	WP_001315909.1	hypothetical protein	-
C5F73_RS00465	93530..94510	-	981	WP_000019440.1	IS5-like element ISKpn26 family transposase	-
C5F73_RS00470	94834..95679	-	846	WP_001518322.1	DUF4942 domain-containing protein	-
C5F73_RS00475	95764..95961	-	198	WP_000839251.1	DUF957 domain-containing protein	-
C5F73_RS00480	95973..96461	-	489	WP_001518318.1	hypothetical protein	-
C5F73_RS00485	96458..96835	-	378	WP_001518317.1	type IV toxin-antitoxin system toxin CbtA	Toxin
C5F73_RS00490	96924..97292	-	369	WP_024187829.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
C5F73_RS00495	97371..97592	-	222	WP_000692312.1	DUF987 domain-containing protein	-
C5F73_RS00500	97661..98137	-	477	WP_001518017.1	RadC family protein	-
C5F73_RS00505	98153..98626	-	474	WP_001518016.1	antirestriction protein	-
C5F73_RS00510	98720..98965	-	246	WP_001518015.1	hypothetical protein	-
C5F73_RS00515	98965..99786	-	822	WP_001518315.1	DUF945 domain-containing protein	-
C5F73_RS26765	99887..100096	-	210	Protein_103	DUF905 family protein	-
C5F73_RS00525	100197..100652	-	456	WP_001518314.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	93530..94510	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13964.02 Da
• Isoelectric Point: 8.9341

>T96659 WP_001518317.1 NZ_CP026932:c96835-96458 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKYPEAKR

Nucleotide

• Length: 378 bp

>T96659 NZ_CP026932:c96835-96458 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGTATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13631.41 Da
• Isoelectric Point: 6.2044

>AT96659 WP_024187829.1 NZ_CP026932:c97292-96924 [Escherichia coli]
VSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT96659 NZ_CP026932:c97292-96924 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA