Detailed information of TA system TA096652

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 61965..62590
• Replicon: plasmid pCFS3246-1
• Accession: NZ_CP026930
• Organism: Escherichia coli strain CFS3246

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C4962_RS23505
• Protein ID: WP_000911314.1
• Coordinates: 62192..62590 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: C4962_RS23500
• Protein ID: WP_000450532.1
• Coordinates: 61965..62192 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4962_RS23465	57241..57945	-	705	WP_001067845.1	IS6-like element IS26 family transposase	-
C4962_RS23475	58233..59362	+	1130	WP_085959879.1	IS3-like element IS1133 family transposase	-
C4962_RS23480	59469..60272	+	804	WP_001082319.1	aminoglycoside O-phosphotransferase APH(3'')-Ib	-
C4962_RS23485	60272..61108	+	837	WP_000480968.1	aminoglycoside O-phosphotransferase APH(6)-Id	-
C4962_RS23490	61012..61215	+	204	Protein_70	mobilization protein (MobL-like)	-
C4962_RS23495	61213..61883	-	671	Protein_71	conjugative relaxase	-
C4962_RS23500	61965..62192	+	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin
C4962_RS23505	62192..62590	+	399	WP_000911314.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C4962_RS23510	62599..64752	-	2154	WP_001375140.1	type IV conjugative transfer system coupling protein TraD	-
C4962_RS23515	65005..65736	-	732	WP_000850426.1	complement resistance protein TraT	-
C4962_RS23520	65750..66259	-	510	WP_000628103.1	conjugal transfer entry exclusion protein TraS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / aph(3'')-Ib / aph(6)-Id	estIa / paa	1..129225	129225
	-	inside	IScluster/Tn	blaTEM-1B / aph(3'')-Ib / aph(6)-Id	-	46524..61108	14584

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14814.12 Da
• Isoelectric Point: 7.8604

>T96652 WP_000911314.1 NZ_CP026930:62192-62590 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELALQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T96652 NZ_CP026930:62192-62590 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGTGTCAGGGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGCATCAGTTCGGTCACTCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGACGTTCTGGATTACGACGCTGCTGCTGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCTTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGTTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT96652 WP_000450532.1 NZ_CP026930:61965-62192 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT96652 NZ_CP026930:61965-62192 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCTGCCGGAGAGACATGGGACGAATGGTTCGACGGAA
ACAGTGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCTGGTATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5