Detailed information of TA system TA096651

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 54679..55238
• Replicon: plasmid pCFS3246-1
• Accession: NZ_CP026930
• Organism: Escherichia coli strain CFS3246

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: C4962_RS23445
• Protein ID: WP_000421257.1
• Coordinates: 54679..54954 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: C4962_RS23450
• Protein ID: WP_001178089.1
• Coordinates: 54954..55238 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4962_RS23405	50050..50754	-	705	WP_001067845.1	IS6-like element IS26 family transposase	-
C4962_RS23410	50817..51041	-	225	Protein_55	DUF4158 domain-containing protein	-
C4962_RS23415	51205..51762	+	558	WP_001235713.1	recombinase family protein	-
C4962_RS23420	51869..52573	+	705	WP_001067845.1	IS6-like element IS26 family transposase	-
C4962_RS23425	52666..53013	-	348	WP_000142451.1	hypothetical protein	-
C4962_RS23430	53142..53477	+	336	WP_001080730.1	colicin 1A immunity protein	-
C4962_RS23435	53499..53858	-	360	WP_085452786.1	colicin	-
C4962_RS23440	53932..54629	+	698	Protein_61	IS1 family transposase	-
C4962_RS23445	54679..54954	-	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4962_RS23450	54954..55238	-	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
C4962_RS23455	55638..55851	-	214	Protein_64	transposase	-
C4962_RS23460	56181..57041	-	861	WP_000027057.1	class A broad-spectrum beta-lactamase TEM-1	-
C4962_RS23465	57241..57945	-	705	WP_001067845.1	IS6-like element IS26 family transposase	-
C4962_RS23475	58233..59362	+	1130	WP_085959879.1	IS3-like element IS1133 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / aph(3'')-Ib / aph(6)-Id	estIa / paa	1..129225	129225
	-	inside	IScluster/Tn	blaTEM-1B / aph(3'')-Ib / aph(6)-Id	-	46524..61108	14584

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T96651 WP_000421257.1 NZ_CP026930:c54954-54679 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T96651 NZ_CP026930:c54954-54679 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT96651 WP_001178089.1 NZ_CP026930:c55238-54954 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT96651 NZ_CP026930:c55238-54954 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: S1PMX5

Antitoxin

• AlphaFold DB: A0A731IHA8