Detailed information of TA system TA096641

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3726425..3727119
• Replicon: chromosome
• Accession: NZ_CP026929
• Organism: Escherichia coli strain CFS3246

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A2A3UTQ9
• Locus tag: C4962_RS18085
• Protein ID: WP_001094399.1
• Coordinates: 3726425..3726793 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: C4962_RS18090
• Protein ID: WP_077249034.1
• Coordinates: 3726814..3727119 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4962_RS18065	3721598..3722554	+	957	WP_000643333.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-
C4962_RS18070	3722551..3724749	+	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
C4962_RS18075	3724759..3725715	+	957	WP_000121359.1	molybdenum cofactor insertion chaperone PaoD	-
C4962_RS18080	3725694..3726104	+	411	WP_001111348.1	hypothetical protein	-
C4962_RS18085	3726425..3726793	-	369	WP_001094399.1	TA system toxin CbtA family protein	Toxin
C4962_RS18090	3726814..3727119	-	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C4962_RS18095	3727223..3727867	-	645	WP_000086755.1	hypothetical protein	-
C4962_RS18100	3727886..3728107	-	222	WP_000692315.1	DUF987 domain-containing protein	-
C4962_RS18105	3728176..3728652	-	477	WP_001186742.1	RadC family protein	-
C4962_RS18110	3728667..3729152	-	486	WP_000214318.1	antirestriction protein	-
C4962_RS18115	3729243..3730061	-	819	WP_001234748.1	DUF945 domain-containing protein	-
C4962_RS18120	3730152..3730385	-	234	WP_001117568.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	3699935..3742290	42355

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13568.73 Da
• Isoelectric Point: 7.7465

>T96641 WP_001094399.1 NZ_CP026929:c3726793-3726425 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
TSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T96641 NZ_CP026929:c3726793-3726425 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCTCCGGCGGCGAAGCCCTGTCCGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTTTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATTG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
ACCTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGTTGATGAGCCGGTG
TTCTTACAGAGATGTAAGTAATATTGTTCTTAGCCGATCACGGTTGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT96641 WP_077249034.1 NZ_CP026929:c3727119-3726814 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT96641 NZ_CP026929:c3727119-3726814 [Escherichia coli]
CTGGTGGGGCTACCCTACACCGTGACGCCCTGTTCTGGCACCCGTCTGGTGCAGGAGGGCAACCGACTGTACTATTTGGC
TGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGCTGGATGTCGTATTTCCACATTTTATCAGTC
AGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTTTCACCCTGTACCACAACGGTTTCACC
TGCGAAGCCGATACCCTTGGTAGTTGCGGCTATGTATACATCGCCATTTATCCCACTCAGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A2A3UTQ9