Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3542990..3543608 Replicon chromosome
Accession NZ_CP026929
Organism Escherichia coli strain CFS3246

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C4962_RS17225 Protein ID WP_001291435.1
Coordinates 3543390..3543608 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C4962_RS17220 Protein ID WP_000344800.1
Coordinates 3542990..3543364 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4962_RS17210 3538079..3539272 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
C4962_RS17215 3539295..3542444 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
C4962_RS17220 3542990..3543364 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C4962_RS17225 3543390..3543608 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
C4962_RS17230 3543780..3544331 + 552 WP_000102564.1 maltose O-acetyltransferase -
C4962_RS17235 3544447..3544917 + 471 WP_000136192.1 YlaC family protein -
C4962_RS17240 3545081..3546631 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C4962_RS17245 3546673..3547026 - 354 WP_000878140.1 DUF1428 domain-containing protein -
C4962_RS17255 3547327..3547716 + 390 WP_001300427.1 DNA base-flipping protein -
C4962_RS17260 3547747..3548319 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T96640 WP_001291435.1 NZ_CP026929:3543390-3543608 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T96640 NZ_CP026929:3543390-3543608 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT96640 WP_000344800.1 NZ_CP026929:3542990-3543364 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT96640 NZ_CP026929:3542990-3543364 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References