Detailed information of TA system TA096628

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1889681..1890516
• Replicon: chromosome
• Accession: NZ_CP026929
• Organism: Escherichia coli strain CFS3246

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1X9TM58
• Locus tag: C4962_RS08985
• Protein ID: WP_000854761.1
• Coordinates: 1889681..1890058 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: C4962_RS08990
• Protein ID: WP_001295723.1
• Coordinates: 1890148..1890516 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4962_RS08950	1885201..1885674	+	474	WP_001105385.1	DNA gyrase inhibitor SbmC	-
C4962_RS08955	1885873..1886931	+	1059	WP_001200892.1	FUSC family protein	-
C4962_RS08960	1887103..1887432	+	330	WP_000450409.1	DUF496 family protein	-
C4962_RS08965	1887533..1887715	-	183	WP_024167529.1	ethanolamine utilization protein	-
C4962_RS08970	1887728..1887898	-	171	Protein_1756	propanediol utilization protein	-
C4962_RS08975	1888169..1889197	-	1029	Protein_1757	IS110 family transposase	-
C4962_RS24365	1889356..1889469	-	114	WP_001161660.1	DUF957 domain-containing protein	-
C4962_RS08980	1889482..1889684	-	203	Protein_1759	hypothetical protein	-
C4962_RS08985	1889681..1890058	-	378	WP_000854761.1	TA system toxin CbtA family protein	Toxin
C4962_RS08990	1890148..1890516	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C4962_RS08995	1890679..1890900	-	222	WP_000692345.1	DUF987 domain-containing protein	-
C4962_RS09000	1890963..1891439	-	477	WP_001186711.1	RadC family protein	-
C4962_RS09005	1891454..1891927	-	474	WP_000855059.1	antirestriction protein	-
C4962_RS09010	1892269..1893087	-	819	WP_001234729.1	DUF945 domain-containing protein	-
C4962_RS09015	1893242..1893400	-	159	WP_001323397.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14205.25 Da
• Isoelectric Point: 7.3249

>T96628 WP_000854761.1 NZ_CP026929:c1890058-1889681 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T96628 NZ_CP026929:c1890058-1889681 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT96628 WP_001295723.1 NZ_CP026929:c1890516-1890148 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT96628 NZ_CP026929:c1890516-1890148 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TM58

Antitoxin

• AlphaFold DB: S1NM52