Detailed information of TA system TA096624

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 831201..831996
• Replicon: chromosome
• Accession: NZ_CP026929
• Organism: Escherichia coli strain CFS3246

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: C4962_RS04035
• Protein ID: WP_059338733.1
• Coordinates: 831201..831575 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A067H947
• Locus tag: C4962_RS04040
• Protein ID: WP_001285415.1
• Coordinates: 831622..831996 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4962_RS04000	826265..826864	+	600	WP_001255034.1	type II secretion system minor pseudopilin GspJ	-
C4962_RS04005	826867..827844	+	978	WP_000633209.1	type II secretion system minor pseudopilin GspK	-
C4962_RS04010	827841..829019	+	1179	WP_000094991.1	type II secretion system protein GspL	-
C4962_RS04015	829021..829557	+	537	WP_000942785.1	GspM family type II secretion system protein YghD	-
C4962_RS04020	829838..830410	-	573	WP_001290246.1	DUF4942 domain-containing protein	-
C4962_RS04025	830507..830704	-	198	WP_000839281.1	DUF957 domain-containing protein	-
C4962_RS04030	830716..831204	-	489	WP_000777545.1	hypothetical protein	-
C4962_RS04035	831201..831575	-	375	WP_059338733.1	TA system toxin CbtA family protein	Toxin
C4962_RS04040	831622..831996	-	375	WP_001285415.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C4962_RS04045	832159..832380	-	222	WP_000692329.1	DUF987 domain-containing protein	-
C4962_RS04050	832443..832919	-	477	WP_001186726.1	RadC family protein	-
C4962_RS04055	832935..833420	-	486	WP_000849582.1	antirestriction protein	-
C4962_RS04060	833475..834293	-	819	WP_001234620.1	DUF945 domain-containing protein	-
C4962_RS04065	834393..834626	-	234	WP_001119729.1	DUF905 family protein	-
C4962_RS04070	834705..835160	-	456	WP_072645706.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14074.11 Da
• Isoelectric Point: 8.7452

>T96624 WP_059338733.1 NZ_CP026929:c831575-831201 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T96624 NZ_CP026929:c831575-831201 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13663.32 Da
• Isoelectric Point: 5.8640

>AT96624 WP_001285415.1 NZ_CP026929:c831996-831622 [Escherichia coli]
VSDTHPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT96624 NZ_CP026929:c831996-831622 [Escherichia coli]
GTGTCAGACACACACCCCGGGACAACGCATCCCGACAACAACAACGACCGCCCCTGGTGGGGGCTCCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGGCAGT
TTAGCGACGCGGATGCGTATCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A067H947