Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 4517002..4517618 Replicon chromosome
Accession NZ_CP026881
Organism Pseudomonas sp. LG1D9

Toxin (Protein)


Gene name hicA Uniprot ID A0A1S1UW78
Locus tag C5F71_RS21110 Protein ID WP_029292612.1
Coordinates 4517002..4517190 (+) Length 63 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7Y1DEL1
Locus tag C5F71_RS21115 Protein ID WP_029292614.1
Coordinates 4517214..4517618 (+) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5F71_RS21080 4512084..4512950 + 867 WP_104911862.1 alpha-D-ribose 1-methylphosphonate 5-phosphate C-P-lyase PhnJ -
C5F71_RS21085 4512911..4513720 + 810 WP_166647843.1 phosphonate C-P lyase system protein PhnK -
C5F71_RS21090 4513720..4514439 + 720 WP_104911864.1 phosphonate C-P lyase system protein PhnL -
C5F71_RS21095 4514429..4515574 + 1146 WP_104911865.1 alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase -
C5F71_RS21100 4515574..4516140 + 567 WP_104911866.1 phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN -
C5F71_RS21105 4516131..4516880 + 750 WP_092234623.1 phosphonate metabolism protein PhnP -
C5F71_RS21110 4517002..4517190 + 189 WP_029292612.1 type II toxin-antitoxin system HicA family toxin Toxin
C5F71_RS21115 4517214..4517618 + 405 WP_029292614.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C5F71_RS21120 4517764..4518960 - 1197 WP_169874588.1 MFS transporter -
C5F71_RS21125 4519190..4520194 - 1005 WP_104911867.1 sulfate ABC transporter substrate-binding protein -
C5F71_RS21130 4520288..4521112 - 825 WP_029292622.1 ion transporter -
C5F71_RS21135 4521144..4522019 - 876 WP_104911868.1 urea transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 63 a.a.        Molecular weight: 7120.29 Da        Isoelectric Point: 11.5133

>T96538 WP_029292612.1 NZ_CP026881:4517002-4517190 [Pseudomonas sp. LG1D9]
VQSRLLIKELQAAGWVLDRVTGSHHIFTHRYNPYTIPVPHPKKDLPLGTVRSIRQRAGLFSF

Download         Length: 189 bp

>T96538 NZ_CP026881:4517002-4517190 [Pseudomonas sp. LG1D9]
GTGCAAAGCAGGCTATTGATCAAGGAACTGCAAGCGGCGGGCTGGGTGCTGGATCGGGTCACGGGCAGCCATCATATTTT
CACGCATCGCTATAACCCGTACACGATACCGGTGCCCCATCCCAAGAAGGACCTGCCCTTGGGCACCGTGCGAAGTATCA
GGCAGCGTGCCGGGCTGTTTTCGTTCTAG

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14742.74 Da        Isoelectric Point: 4.6115

>AT96538 WP_029292614.1 NZ_CP026881:4517214-4517618 [Pseudomonas sp. LG1D9]
MQYPICIEWGDDFTATGIQIPDIPGAVTAGDSFEEAYNAAVEVAHIMLQEMASEGRAIPMPTSVAAHHGNEEYAGMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHKVKSRSSFLADAALEKLVRS

Download         Length: 405 bp

>AT96538 NZ_CP026881:4517214-4517618 [Pseudomonas sp. LG1D9]
ATGCAATACCCAATCTGTATCGAATGGGGCGATGACTTTACCGCCACCGGTATCCAGATCCCCGATATTCCAGGTGCGGT
GACCGCCGGGGACAGTTTTGAAGAGGCTTACAACGCGGCAGTGGAAGTGGCGCACATCATGCTGCAAGAGATGGCCAGTG
AAGGCCGGGCGATTCCCATGCCGACCTCGGTCGCGGCGCACCACGGCAACGAGGAATACGCCGGGATGGGCTGGGGCATG
CTGGAGCTCGATATTTCGCCTTACCTGGGCAAGACCGAGAAGGTCAATGTGACCTTGCCAGGGTATGTGATCCAGCGCAT
TGACCGGTATGTACGCGAGCACAAGGTGAAGAGCCGCTCATCATTCCTGGCGGATGCGGCGTTGGAGAAGTTGGTGCGTT
CTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1S1UW78

Antitoxin

Source ID Structure
AlphaFold DB A0A7Y1DEL1

References