96537

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 4194313..4194929 Replicon chromosome
Accession NZ_CP026881
Organism Pseudomonas sp. LG1D9

Toxin (Protein)


Gene name hicA Uniprot ID A0A7Y1DDS6
Locus tag C5F71_RS19610 Protein ID WP_029299101.1
Coordinates 4194747..4194929 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1S1UT55
Locus tag C5F71_RS19605 Protein ID WP_070995801.1
Coordinates 4194313..4194723 (-) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5F71_RS19580 4190565..4191053 - 489 WP_029299107.1 DMT family transporter -
C5F71_RS19585 4191122..4192048 + 927 WP_104911763.1 LysR family transcriptional regulator -
C5F71_RS19590 4192114..4192755 + 642 WP_029299105.1 LysE family translocator -
C5F71_RS19595 4192874..4193401 + 528 WP_029299104.1 DUF4142 domain-containing protein -
C5F71_RS29080 4193558..4193704 + 147 WP_166647832.1 hypothetical protein -
C5F71_RS19600 4193710..4194210 - 501 WP_029299103.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
C5F71_RS19605 4194313..4194723 - 411 WP_070995801.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C5F71_RS19610 4194747..4194929 - 183 WP_029299101.1 type II toxin-antitoxin system HicA family toxin Toxin
C5F71_RS19615 4195961..4196932 + 972 WP_104911764.1 hypothetical protein -
C5F71_RS28960 4197208..4197435 + 228 WP_158682873.1 hypothetical protein -
C5F71_RS19620 4197335..4199836 + 2502 WP_177410409.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-128)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6773.94 Da        Isoelectric Point: 10.8600

>T96537 WP_029299101.1 NZ_CP026881:c4194929-4194747 [Pseudomonas sp. LG1D9]
VDSRYLIGHIVADGWYLARVRGSHHHFKHPTKPGLVTIPHPRKELLDKTAKSILKQALLG

Download         Length: 183 bp

>T96537 NZ_CP026881:c4194929-4194747 [Pseudomonas sp. LG1D9]
GTGGATAGCCGATATTTGATAGGCCATATCGTTGCAGACGGCTGGTATCTGGCACGCGTCAGGGGCAGCCATCATCATTT
CAAACATCCAACCAAGCCTGGGCTGGTGACCATTCCCCATCCCCGGAAAGAGCTGTTGGACAAGACCGCCAAGAGCATTT
TGAAACAAGCTCTGCTCGGTTGA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 14811.77 Da        Isoelectric Point: 4.5025

>AT96537 WP_070995801.1 NZ_CP026881:c4194723-4194313 [Pseudomonas sp. LG1D9]
MLYPIAISTGDDDHAWGVEVPDIPGCFSAGDDLDDAMAMAREAIEGHFELLAEDGAPIPSAQKVTHHAANPLYAGCIWAV
VDIDVTKYLGKAQKLNITLPGYLLNRIDEYVLHHPEEKSRSGFLASAALKVLQQER

Download         Length: 411 bp

>AT96537 NZ_CP026881:c4194723-4194313 [Pseudomonas sp. LG1D9]
ATGCTGTATCCGATTGCAATCTCAACGGGTGACGACGACCACGCCTGGGGAGTGGAAGTCCCCGACATCCCTGGGTGTTT
CTCTGCCGGAGATGACCTGGATGACGCCATGGCCATGGCACGGGAGGCCATAGAAGGTCACTTCGAGCTGCTGGCGGAAG
ATGGCGCGCCCATTCCCAGCGCGCAAAAAGTCACCCACCACGCGGCCAACCCTCTGTACGCCGGCTGCATCTGGGCGGTA
GTGGACATTGATGTGACCAAGTACCTGGGCAAGGCGCAAAAACTCAACATCACCTTGCCGGGTTATCTGCTCAACCGCAT
TGATGAATACGTGCTGCATCACCCGGAAGAAAAAAGCCGCTCCGGGTTCCTCGCTTCGGCAGCGCTGAAAGTGCTGCAAC
AAGAGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Y1DDS6

Antitoxin

Source ID Structure
AlphaFold DB A0A1S1UT55

References