Detailed information of TA system TA096535

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2594347..2595896
• Replicon: chromosome
• Accession: NZ_CP026881
• Organism: Pseudomonas sp. LG1D9

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: C5F71_RS12530
• Protein ID: WP_104912616.1
• Coordinates: 2594622..2595896 (-)
• Length: 425 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A1S1UPJ9
• Locus tag: C5F71_RS12525
• Protein ID: WP_029290025.1
• Coordinates: 2594347..2594625 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5F71_RS12495	2589795..2590442	+	648	WP_104911226.1	FMN-binding negative transcriptional regulator	-
C5F71_RS12500	2590429..2590875	+	447	WP_092232253.1	GNAT family N-acetyltransferase	-
C5F71_RS12505	2590912..2591583	+	672	WP_092232256.1	GNAT family N-acetyltransferase	-
C5F71_RS12510	2591684..2592583	-	900	WP_092232259.1	homocysteine S-methyltransferase family protein	-
C5F71_RS12515	2592695..2593516	+	822	WP_104911227.1	ABC transporter substrate-binding protein	-
C5F71_RS12520	2593513..2594343	+	831	WP_029290023.1	amino acid ABC transporter permease	-
C5F71_RS12525	2594347..2594625	-	279	WP_029290025.1	helix-turn-helix domain-containing protein	Antitoxin
C5F71_RS12530	2594622..2595896	-	1275	WP_104912616.1	HipA domain-containing protein	Toxin
C5F71_RS29075	2595795..2596049	+	255	WP_177410388.1	hypothetical protein	-
C5F71_RS12535	2596238..2597506	+	1269	WP_104911228.1	autotransporter outer membrane beta-barrel domain-containing protein	-
C5F71_RS12540	2597572..2599380	-	1809	WP_029290033.1	sodium-extruding oxaloacetate decarboxylase subunit alpha	-
C5F71_RS12545	2599392..2600807	-	1416	WP_029290035.1	acetyl-CoA carboxylase biotin carboxylase subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-351)

Antitoxin

• Cro/C1-type helix-turn-helix domain (27-80)

Sequences

Toxin

Protein

• Length: 425 a.a.
• Molecular weight: 46789.21 Da
• Isoelectric Point: 6.2725

>T96535 WP_104912616.1 NZ_CP026881:c2595896-2594622 [Pseudomonas sp. LG1D9]
MRLEFSEPQKGFDGPCRFHYRQDYLVEHLGDIGQGFCSAVSGRFPLEWDVTFLKTAPAFLHDIAPAGAAKRFLLQRMGAG
KPDDVSVDLFLLGRCTPSPIGNLRIKESAQALGVRPAVGFSRHDVVSRDNGFLEYAYEQGAAIGGATGAGGEAPKLLMAE
HRDGLLYPDAVLPDAEVAQHWFIKFARNGATLNDQAILRSEFHYYKALQALGIETVAAQGLALEEAHKPSLWMQRFDRNV
TSAGVERLAVESIYSLAGVVTPGSYLDHLHVVQMLAEHWRQVGQGDGVADLVAEYLRRDLLNKILGNSDNHGRNTSIIRE
RNSMRLAPIYDLAPMVMDAEGITRTTKWPKGLEVAGEIDWQGVCGALEGLVDPQQAFERLRADAEHLRALPDILVNSGLP
QETWKHPAIALGKLDQRLQQWGLR

Nucleotide

• Length: 1275 bp

>T96535 NZ_CP026881:c2595896-2594622 [Pseudomonas sp. LG1D9]
ATGCGGCTGGAGTTTTCCGAGCCTCAAAAAGGTTTTGATGGACCCTGTCGGTTTCATTATCGGCAGGACTATCTCGTGGA
GCACCTTGGCGACATTGGACAAGGTTTTTGTTCTGCGGTGAGTGGGCGGTTTCCTCTGGAGTGGGATGTCACCTTCCTCA
AGACGGCTCCTGCCTTCTTGCATGACATTGCACCGGCGGGTGCGGCGAAACGCTTCCTGCTGCAGCGAATGGGCGCGGGA
AAGCCGGACGATGTCAGCGTCGACTTGTTTCTGCTGGGCCGCTGCACGCCGTCGCCTATTGGCAACCTGCGCATCAAGGA
GTCCGCCCAGGCATTGGGTGTCAGGCCCGCCGTGGGCTTCAGCCGTCACGATGTGGTCAGTCGCGACAATGGCTTCCTGG
AATACGCCTATGAGCAAGGCGCAGCCATTGGTGGCGCGACCGGGGCGGGCGGCGAAGCACCGAAGTTGCTGATGGCCGAG
CATCGCGACGGTCTGCTGTATCCGGACGCTGTTCTTCCCGACGCCGAGGTCGCCCAACACTGGTTTATCAAGTTTGCCCG
CAATGGCGCGACCCTCAACGACCAAGCGATCCTCAGGAGCGAATTTCACTACTACAAGGCGCTGCAGGCGCTGGGTATCG
AAACCGTCGCCGCCCAAGGGCTGGCGTTGGAAGAGGCGCACAAACCCAGCCTGTGGATGCAGCGTTTTGACCGTAACGTC
ACGTCGGCGGGCGTCGAGCGGCTGGCCGTCGAGTCGATCTATTCACTGGCGGGGGTCGTTACGCCGGGCAGCTATTTGGA
TCATCTGCACGTCGTGCAGATGCTCGCCGAACACTGGCGACAGGTCGGGCAGGGTGACGGCGTTGCCGACTTGGTCGCCG
AGTATTTGCGGCGAGACTTGCTCAACAAAATCCTCGGTAACTCGGATAACCACGGGCGTAACACCTCGATTATCCGCGAG
CGCAACTCCATGCGTCTGGCGCCGATTTATGACTTGGCCCCCATGGTCATGGATGCCGAAGGCATCACCCGCACCACCAA
GTGGCCCAAGGGCCTGGAAGTGGCCGGTGAAATTGACTGGCAAGGGGTTTGCGGCGCACTGGAGGGCCTGGTTGATCCGC
AGCAAGCCTTTGAACGACTGCGCGCCGACGCCGAACACTTGCGGGCGTTGCCGGACATTCTGGTGAACAGTGGTTTGCCC
CAGGAAACCTGGAAGCACCCCGCGATCGCGCTCGGCAAACTGGATCAACGTCTACAGCAATGGGGGCTGCGATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10197.95 Da
• Isoelectric Point: 11.9342

>AT96535 WP_029290025.1 NZ_CP026881:c2594625-2594347 [Pseudomonas sp. LG1D9]
MTVSMDTRALLIQGIEQGLAQGTLQIGEAVRRLRVEVTGLHQSQFARMCKISVRTLVHIEHGEGNQTLKSLNAVFRPFGL
QMGVVRVRRSLG

Nucleotide

• Length: 279 bp

>AT96535 NZ_CP026881:c2594625-2594347 [Pseudomonas sp. LG1D9]
ATGACTGTCAGCATGGATACACGCGCCCTGCTGATCCAGGGCATCGAGCAGGGCCTGGCCCAGGGCACCCTGCAAATCGG
TGAAGCGGTACGCCGTCTTCGCGTTGAAGTCACGGGCCTGCACCAGAGCCAATTTGCCAGGATGTGCAAGATTTCCGTGC
GCACGCTGGTGCATATCGAGCATGGCGAGGGCAATCAGACGCTCAAGTCGTTGAACGCTGTGTTCCGTCCGTTCGGCTTG
CAGATGGGGGTGGTCAGGGTGCGACGTTCGCTGGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1S1UPJ9