Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 4218730..4219313 Replicon chromosome
Accession NZ_CP026878
Organism Shigella dysenteriae strain 07-3308

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag C1P62_RS22990 Protein ID WP_000254738.1
Coordinates 4218978..4219313 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag C1P62_RS22985 Protein ID WP_000581937.1
Coordinates 4218730..4218978 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P62_RS22975 4215069..4216370 + 1302 WP_000046834.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
C1P62_RS22980 4216418..4218652 + 2235 WP_000226815.1 GTP pyrophosphokinase -
C1P62_RS22985 4218730..4218978 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
C1P62_RS22990 4218978..4219313 + 336 WP_000254738.1 endoribonuclease MazF Toxin
C1P62_RS22995 4219384..4220175 + 792 WP_001071643.1 nucleoside triphosphate pyrophosphohydrolase -
C1P62_RS23000 4220398..4222035 + 1638 WP_134806332.1 CTP synthase (glutamine hydrolyzing) -
C1P62_RS23005 4222123..4223421 + 1299 WP_000036723.1 phosphopyruvate hydratase -
C1P62_RS23010 4223481..4223831 - 351 Protein_4339 YgcG family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T96528 WP_000254738.1 NZ_CP026878:4218978-4219313 [Shigella dysenteriae]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T96528 NZ_CP026878:4218978-4219313 [Shigella dysenteriae]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT96528 WP_000581937.1 NZ_CP026878:4218730-4218978 [Shigella dysenteriae]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT96528 NZ_CP026878:4218730-4218978 [Shigella dysenteriae]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACTTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References