Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 8872..9431 Replicon plasmid pMS7163B
Accession NZ_CP026855
Organism Escherichia coli strain MS7163

Toxin (Protein)


Gene name relE Uniprot ID S1PMX5
Locus tag MS7163_RS27550 Protein ID WP_000421257.1
Coordinates 8872..9147 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag MS7163_RS27555 Protein ID WP_001178089.1
Coordinates 9147..9431 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MS7163_RS27500 4052..4678 + 627 WP_000587689.1 ParA family plasmid-partitioning AAA ATPase -
MS7163_RS28505 4675..4977 + 303 WP_001245884.1 hypothetical protein -
MS7163_RS27515 5488..6834 - 1347 WP_001132022.1 hypothetical protein -
MS7163_RS27525 7188..7790 + 603 WP_000517694.1 hypothetical protein -
MS7163_RS28510 7846..8028 + 183 Protein_10 hypothetical protein -
MS7163_RS28515 8028..8294 + 267 WP_000627831.1 hypothetical protein -
MS7163_RS27545 8485..8766 - 282 WP_001105066.1 hypothetical protein -
MS7163_RS27550 8872..9147 - 276 WP_000421257.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MS7163_RS27555 9147..9431 - 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
MS7163_RS27575 10334..11206 - 873 WP_000729437.1 incFII family plasmid replication initiator RepA -
MS7163_RS27590 11909..12550 + 642 WP_000335849.1 transcription termination factor NusG -
MS7163_RS27595 12691..13353 + 663 WP_000154142.1 hypothetical protein -
MS7163_RS28520 13620..13763 + 144 WP_072105535.1 hypothetical protein -
MS7163_RS27605 13769..14086 + 318 WP_001330559.1 PilI type IV pilus biogenesis protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / dfrA14 / aph(6)-Id - 1..84078 84078


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 9.9324

>T96511 WP_000421257.1 NZ_CP026855:c9147-8872 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T96511 NZ_CP026855:c9147-8872 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCAGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT96511 WP_001178089.1 NZ_CP026855:c9431-9147 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT96511 NZ_CP026855:c9431-9147 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PMX5

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References