Detailed information of TA system TA096510

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 79900..80348
• Replicon: plasmid pMS7163A
• Accession: NZ_CP026854
• Organism: Escherichia coli strain MS7163

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: MS7163_RS27170
• Protein ID: WP_000970009.1
• Coordinates: 80166..80348 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: MS7163_RS27165
• Protein ID: WP_000079941.1
• Coordinates: 79900..80169 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS7163_RS28305	75231..75467	+	237	WP_001323890.1	colicin V immunity protein	-
MS7163_RS27150	75445..75756	+	312	WP_001259758.1	colicin V	-
MS7163_RS27155	75926..78040	-	2115	WP_001183604.1	peptidase domain-containing ABC transporter	-
MS7163_RS27160	78015..79289	-	1275	WP_000489608.1	HlyD family secretion protein	-
MS7163_RS27165	79900..80169	+	270	WP_000079941.1	hypothetical protein	Antitoxin
MS7163_RS27170	80166..80348	+	183	WP_000970009.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MS7163_RS27175	80447..80755	+	309	WP_000377483.1	hypothetical protein	-
MS7163_RS27185	81315..82349	+	1035	WP_001190234.1	3-deoxy-7-phosphoheptulonate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroN / iroE / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA	1..133843	133843

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6917.00 Da
• Isoelectric Point: 4.2884

>T96510 WP_000970009.1 NZ_CP026854:80166-80348 [Escherichia coli]
MMEIFWTMLASQDRNRIREYIAEQNLIAAIELDERIGCSASLLFSLSFISVQVHDNIITV

Nucleotide

• Length: 183 bp

>T96510 NZ_CP026854:80166-80348 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAATCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
AGCCGCCATTGAACTGGACGAACGGATTGGTTGTTCAGCCAGCCTCCTGTTCTCCCTTTCTTTTATCAGTGTACAGGTGC
ATGACAACATAATCACGGTATGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT96510 WP_000079941.1 NZ_CP026854:79900-80169 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT96510 NZ_CP026854:79900-80169 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAAGTCGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5