Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 67841..68388 Replicon plasmid pMS7163A
Accession NZ_CP026854
Organism Escherichia coli strain MS7163

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag MS7163_RS27085 Protein ID WP_000969996.1
Coordinates 68107..68388 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E1QMW3
Locus tag MS7163_RS27080 Protein ID WP_000079941.1
Coordinates 67841..68110 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MS7163_RS27030 63115..63861 + 747 WP_000205759.1 conjugal transfer pilus acetylase TraX -
MS7163_RS27035 63916..64476 + 561 WP_000139359.1 fertility inhibition protein FinO -
MS7163_RS27040 64614..64826 + 213 WP_001311693.1 hypothetical protein -
MS7163_RS27045 65069..65470 + 402 WP_001233870.1 thermonuclease family protein -
MS7163_RS27050 65515..65775 + 261 WP_000083818.1 replication regulatory protein RepA -
MS7163_RS27060 66001..66075 + 75 WP_001365705.1 RepA leader peptide Tap -
MS7163_RS27065 66068..66925 + 858 WP_000130940.1 incFII family plasmid replication initiator RepA -
MS7163_RS27080 67841..68110 + 270 WP_000079941.1 hypothetical protein Antitoxin
MS7163_RS27085 68107..68388 + 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MS7163_RS27090 68434..69282 + 849 WP_001057989.1 3'-5' exonuclease -
MS7163_RS27095 69468..71348 + 1881 WP_001283335.1 colicin -
MS7163_RS27100 71370..71705 - 336 WP_001080729.1 colicin 1A immunity protein -
MS7163_RS27110 71834..72091 + 258 WP_000142431.1 hypothetical protein -
MS7163_RS27115 72111..72701 - 591 WP_001350923.1 hypothetical protein -
MS7163_RS27120 72698..72958 - 261 WP_000343091.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iroN / iroE / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA 1..133843 133843


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T96509 WP_000969996.1 NZ_CP026854:68107-68388 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T96509 NZ_CP026854:68107-68388 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2057

>AT96509 WP_000079941.1 NZ_CP026854:67841-68110 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT96509 NZ_CP026854:67841-68110 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAAGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A629TXC5

References