Detailed information of TA system TA096506

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4664937..4665771
• Replicon: chromosome
• Accession: NZ_CP026853
• Organism: Escherichia coli strain MS7163

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7MK10
• Locus tag: MS7163_RS24270
• Protein ID: WP_000854820.1
• Coordinates: 4664937..4665314 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: MS7163_RS24275
• Protein ID: WP_001285610.1
• Coordinates: 4665403..4665771 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS7163_RS24245	4661500..4663038	-	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-
MS7163_RS24255	4663873..4664433	-	561	Protein_4448	DUF4942 domain-containing protein	-
MS7163_RS24260	4664518..4664715	-	198	WP_085949158.1	DUF957 domain-containing protein	-
MS7163_RS24265	4664743..4664940	-	198	Protein_4450	hypothetical protein	-
MS7163_RS24270	4664937..4665314	-	378	WP_000854820.1	type IV toxin-antitoxin system toxin CbtA	Toxin
MS7163_RS24275	4665403..4665771	-	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MS7163_RS24280	4665851..4666072	-	222	WP_000692347.1	DUF987 domain-containing protein	-
MS7163_RS24285	4666159..4666635	-	477	WP_001186193.1	RadC family protein	-
MS7163_RS24290	4666650..4667129	-	480	WP_000706978.1	antirestriction protein	-
MS7163_RS24295	4667395..4668213	-	819	WP_001175155.1	DUF945 domain-containing protein	-
MS7163_RS24300	4668303..4668536	-	234	WP_000883181.1	DUF905 family protein	-
MS7163_RS24305	4668542..4669219	-	678	WP_001097565.1	hypothetical protein	-
MS7163_RS24310	4669370..4670050	-	681	WP_001278649.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	papX	4654011..4726763	72752

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14043.12 Da
• Isoelectric Point: 7.8839

>T96506 WP_000854820.1 NZ_CP026853:c4665314-4664937 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNIILGKHPEVKQ

Nucleotide

• Length: 378 bp

>T96506 NZ_CP026853:c4665314-4664937 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAATATTATCCTGGGTAAGCACCCGGAGGTGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT96506 WP_001285610.1 NZ_CP026853:c4665771-4665403 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT96506 NZ_CP026853:c4665771-4665403 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGTACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0E2KXX6