Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 483299..483917 Replicon chromosome
Accession NZ_CP026853
Organism Escherichia coli strain MS7163

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag MS7163_RS02390 Protein ID WP_001291435.1
Coordinates 483299..483517 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag MS7163_RS02395 Protein ID WP_000344800.1
Coordinates 483543..483917 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MS7163_RS02355 478586..479158 + 573 WP_000779831.1 YbaY family lipoprotein -
MS7163_RS02360 479189..479500 - 312 WP_000409908.1 MGMT family protein -
MS7163_RS02370 479879..480232 + 354 WP_000878140.1 DUF1428 domain-containing protein -
MS7163_RS02375 480274..481824 - 1551 WP_001350616.1 cyclic-guanylate-specific phosphodiesterase PdeB -
MS7163_RS02380 481988..482458 - 471 WP_000136192.1 YlaC family protein -
MS7163_RS02385 482574..483125 - 552 WP_000102543.1 maltose O-acetyltransferase -
MS7163_RS02390 483299..483517 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
MS7163_RS02395 483543..483917 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
MS7163_RS02400 484462..487611 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
MS7163_RS02405 487634..488827 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T96479 WP_001291435.1 NZ_CP026853:c483517-483299 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T96479 NZ_CP026853:c483517-483299 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT96479 WP_000344800.1 NZ_CP026853:c483917-483543 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT96479 NZ_CP026853:c483917-483543 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References