Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 33094..33620 Replicon plasmid unnamed1
Accession NZ_CP026851
Organism Enterobacter cloacae complex sp. strain AR_0072

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag AM451_RS23070 Protein ID WP_000323025.1
Coordinates 33094..33381 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID J5W3H0
Locus tag AM451_RS23075 Protein ID WP_004196370.1
Coordinates 33381..33620 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM451_RS23050 (AM451_23055) 29443..29754 - 312 WP_015572115.1 hypothetical protein -
AM451_RS23060 (AM451_23065) 29963..31083 + 1121 WP_085950818.1 IS3-like element ISSen4 family transposase -
AM451_RS24305 31210..31386 - 177 WP_154597702.1 hypothetical protein -
AM451_RS23065 (AM451_23070) 31606..32946 - 1341 WP_015572075.1 ISNCY family transposase -
AM451_RS23070 (AM451_23075) 33094..33381 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
AM451_RS23075 (AM451_23080) 33381..33620 - 240 WP_004196370.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
AM451_RS24590 33645..33743 + 99 Protein_35 hypothetical protein -
AM451_RS23080 (AM451_23085) 33871..34236 + 366 WP_009651956.1 hypothetical protein -
AM451_RS23085 (AM451_23090) 34280..35017 + 738 WP_008460272.1 HupE/UreJ family protein -
AM451_RS23090 (AM451_23095) 35031..35720 + 690 WP_004196322.1 hypothetical protein -
AM451_RS23095 (AM451_23100) 35751..37127 - 1377 WP_004196363.1 chromate efflux transporter -
AM451_RS23100 (AM451_23105) 37084..38061 - 978 WP_004196334.1 chromate resistance protein -
AM451_RS23105 (AM451_23110) 38091..38283 + 193 Protein_41 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..150426 150426


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(3-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T96473 WP_000323025.1 NZ_CP026851:c33381-33094 [Enterobacter cloacae complex sp.]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T96473 NZ_CP026851:c33381-33094 [Enterobacter cloacae complex sp.]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9018.43 Da        Isoelectric Point: 4.3894

>AT96473 WP_004196370.1 NZ_CP026851:c33620-33381 [Enterobacter cloacae complex sp.]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLCNPKPVRVTLDEL

Download         Length: 240 bp

>AT96473 NZ_CP026851:c33620-33381 [Enterobacter cloacae complex sp.]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTTGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4QRH4

References