Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4359135..4359932 Replicon chromosome
Accession NZ_CP026850
Organism Enterobacter cloacae complex sp. strain AR_0072

Toxin (Protein)


Gene name KacT Uniprot ID A0A4Q2R3N2
Locus tag AM451_RS21180 Protein ID WP_032670168.1
Coordinates 4359411..4359932 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID F5RT92
Locus tag AM451_RS21175 Protein ID WP_001303459.1
Coordinates 4359135..4359404 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM451_RS21145 (AM451_21150) 4354675..4355034 + 360 WP_040117469.1 helix-turn-helix domain-containing protein -
AM451_RS24565 4355106..4355207 - 102 Protein_4015 transcriptional regulator -
AM451_RS21150 (AM451_21155) 4355360..4356601 + 1242 WP_047051685.1 bifunctional glucose-1-phosphatase/inositol phosphatase -
AM451_RS21155 (AM451_21160) 4356634..4356861 - 228 WP_006809324.1 YccJ family protein -
AM451_RS21160 (AM451_21165) 4356880..4357476 - 597 WP_006809323.1 NAD(P)H:quinone oxidoreductase -
AM451_RS21165 (AM451_21170) 4357868..4358038 + 171 WP_001273664.1 general stress protein -
AM451_RS21170 (AM451_21175) 4358155..4359060 + 906 WP_047051684.1 DMT family transporter -
AM451_RS21175 (AM451_21180) 4359135..4359404 + 270 WP_001303459.1 DUF1778 domain-containing protein Antitoxin
AM451_RS21180 (AM451_21185) 4359411..4359932 + 522 WP_032670168.1 GNAT family N-acetyltransferase Toxin
AM451_RS21185 (AM451_21190) 4359991..4361313 - 1323 WP_048982825.1 pyrimidine utilization transport protein G -
AM451_RS21190 (AM451_21195) 4361335..4361826 - 492 WP_047052688.1 pyrimidine utilization flavin reductase protein F -
AM451_RS21195 (AM451_21200) 4361839..4362429 - 591 WP_047052687.1 malonic semialdehyde reductase -
AM451_RS21200 (AM451_21205) 4362439..4363239 - 801 WP_047052686.1 pyrimidine utilization protein D -
AM451_RS21205 (AM451_21210) 4363247..4363633 - 387 WP_032670161.1 pyrimidine utilization protein C -
AM451_RS21210 (AM451_21215) 4363645..4364334 - 690 WP_047052685.1 pyrimidine utilization protein B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19437.18 Da        Isoelectric Point: 7.4318

>T96472 WP_032670168.1 NZ_CP026850:4359411-4359932 [Enterobacter cloacae complex sp.]
VANLTIEMLSEGTDYDFGDFDCGEPSLNAFLTEHLVRQHGGRILRGYLLKERDHPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQA

Download         Length: 522 bp

>T96472 NZ_CP026850:4359411-4359932 [Enterobacter cloacae complex sp.]
GTGGCAAATCTCACGATCGAAATGTTATCAGAGGGAACCGATTACGATTTCGGCGATTTCGATTGTGGTGAACCCTCGCT
AAATGCCTTCCTTACCGAGCACCTGGTACGTCAGCACGGCGGGCGTATTTTACGCGGCTACCTGCTTAAAGAACGCGACC
ATCCGCGTGTCCTGGGCTATTACACACTCTCGGGAAGTTGCTTTGAAAAAGCGATGCTTCCTTCAAAGACTCAGCAGCGC
CGCATCCCATACAGCAACGTCCCCAGCGTGACGCTGGGGCGTCTGGCTGTCCATAAAGAACTACAGGGAAACGAGTGGGG
AACAACGCTTGTCACACACGCTATGCGCGTTGTTTATCTGGCGTCGCAGGCTGTTGGCGTCCACGGAATTTTTGTGGATG
CGCTTAACGAACGGGCCAAACGATTTTATCTGAAGCTGGGGTTTATCCCGCTGGCAGGTGAAAACAGCAGTTCGCTGTTC
TTCCCCACGCAATCTATCGAACGACTGTTTGAGCAGGCGTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10248.70 Da        Isoelectric Point: 5.8836

>AT96472 WP_001303459.1 NZ_CP026850:4359135-4359404 [Enterobacter cloacae complex sp.]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Download         Length: 270 bp

>AT96472 NZ_CP026850:4359135-4359404 [Enterobacter cloacae complex sp.]
ATGCCAGCACTCAAAAAACAGCGTATCGATCTTCGCCTGTCGGACGAAGACAAAACAATGATTGAAGAAGCGGCAGCGAT
GACCAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCCGAAGTCATCGAACAGCATCGGCGTC
TCGTATTAAACGAAGCCTCATGGAATGCCGTTATGGATGCCATCGACAATCCGCCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCGAAACGTCTTCGAAACATGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4Q2R3N2

Antitoxin

Source ID Structure
AlphaFold DB A0A7D7BCZ2

References