Detailed information of TA system TA096471

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 4248373..4249286
• Replicon: chromosome
• Accession: NZ_CP026850
• Organism: Enterobacter cloacae complex sp. strain AR_0072

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: AM451_RS20665
• Protein ID: WP_047052345.1
• Coordinates: 4248373..4248846 (-)
• Length: 158 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: AM451_RS20670
• Protein ID: WP_047052347.1
• Coordinates: 4248843..4249286 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM451_RS20645 (AM451_20650)	4244532..4245842	+	1311	WP_047052339.1	type I-F CRISPR-associated protein Csy1	-
AM451_RS20650 (AM451_20655)	4245839..4246774	+	936	WP_104999954.1	type I-F CRISPR-associated protein Csy2	-
AM451_RS20655 (AM451_20660)	4246788..4247786	+	999	WP_047052342.1	type I-F CRISPR-associated protein Csy3	-
AM451_RS20660 (AM451_20665)	4247796..4248350	+	555	WP_047052344.1	type I-F CRISPR-associated endoribonuclease Cas6/Csy4	-
AM451_RS20665 (AM451_20670)	4248373..4248846	-	474	WP_047052345.1	RES domain-containing protein	Toxin
AM451_RS20670 (AM451_20675)	4248843..4249286	-	444	WP_047052347.1	DUF2384 domain-containing protein	Antitoxin
AM451_RS20675 (AM451_20680)	4251238..4251456	-	219	WP_002211347.1	translation initiation factor IF-1	-
AM451_RS20680 (AM451_20685)	4251741..4252445	-	705	WP_047052381.1	leucyl/phenylalanyl-tRNA--protein transferase	-
AM451_RS20685 (AM451_20690)	4252491..4254212	-	1722	WP_003858307.1	cysteine/glutathione ABC transporter ATP-binding protein/permease CydC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (94-144)

Sequences

Toxin

Protein

• Length: 158 a.a.
• Molecular weight: 17570.95 Da
• Isoelectric Point: 5.1151

>T96471 WP_047052345.1 NZ_CP026850:c4248846-4248373 [Enterobacter cloacae complex sp.]
MIFYRLVTGRFASEAWSGSGANQYGGRWNHKGHPAVYVSTSISLASLEILVHVRKDTVLNQYQLFSINIPDDQIDYLDKA
WLPEDWQENPAPVSTMDLGTGWLQANSALALILPSCIIPYENNAILNPLHPAFRKALNSVELLPFDFDTRLAEKTAP

Nucleotide

• Length: 474 bp

>T96471 NZ_CP026850:c4248846-4248373 [Enterobacter cloacae complex sp.]
ATGATTTTTTATCGTCTGGTAACAGGACGTTTTGCCAGCGAAGCGTGGAGTGGAAGTGGTGCAAACCAGTATGGAGGACG
CTGGAATCATAAAGGCCATCCTGCCGTGTATGTCTCCACCTCCATCTCGCTGGCTTCACTGGAAATTCTGGTGCACGTCA
GAAAAGACACCGTGCTGAACCAGTACCAGCTTTTCAGCATTAATATCCCGGATGATCAGATTGATTACCTGGATAAAGCG
TGGCTGCCCGAGGACTGGCAGGAAAATCCGGCGCCGGTATCAACGATGGATCTGGGTACCGGTTGGTTGCAGGCTAACAG
CGCTCTCGCACTCATCTTACCCTCCTGCATCATTCCCTATGAAAACAACGCCATACTTAATCCATTGCATCCGGCATTCC
GCAAAGCACTCAATTCTGTTGAATTACTGCCTTTTGATTTTGATACCCGTCTCGCAGAAAAAACCGCACCCTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16502.77 Da
• Isoelectric Point: 6.4819

>AT96471 WP_047052347.1 NZ_CP026850:c4249286-4248843 [Enterobacter cloacae complex sp.]
MRTWVPAQRSADNTLWRYAGLPASRGMRLIEYLNQGLPVSVLDNIHEWTDMSKAEILRVTGINERNVARRKSTGRTLTPD
ESERVARFVRVLDAAVDYFGSKEEAWNWLQLPVRGLGNVAPVDLIATETGALEVTDLIGRLEHGVFA

Nucleotide

• Length: 444 bp

>AT96471 NZ_CP026850:c4249286-4248843 [Enterobacter cloacae complex sp.]
ATGAGAACATGGGTTCCTGCGCAAAGGTCGGCTGATAACACGCTGTGGCGCTACGCCGGTTTACCTGCAAGCAGAGGCAT
GCGGTTGATTGAATATCTCAATCAAGGATTACCCGTCAGCGTCCTCGACAATATTCACGAGTGGACGGACATGTCGAAAG
CGGAGATTTTACGCGTCACGGGAATTAACGAGCGTAACGTTGCCCGCCGCAAAAGTACGGGCCGGACGCTGACGCCCGAC
GAGAGCGAACGTGTGGCGCGTTTCGTTCGGGTACTGGATGCGGCCGTCGATTATTTCGGCAGTAAAGAAGAGGCATGGAA
CTGGTTGCAGTTACCGGTACGTGGGCTTGGCAATGTTGCACCTGTCGATCTGATTGCCACGGAAACCGGTGCGCTGGAAG
TTACCGATTTGATTGGTCGGCTGGAGCACGGAGTGTTCGCATGA