96470

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3788109..3788729 Replicon chromosome
Accession NZ_CP026850
Organism Enterobacter cloacae complex sp. strain AR_0072

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag AM451_RS18465 Protein ID WP_015571250.1
Coordinates 3788109..3788327 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag AM451_RS18470 Protein ID WP_006809850.1
Coordinates 3788355..3788729 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM451_RS18435 (AM451_18440) 3784121..3784381 + 261 WP_015571255.1 type B 50S ribosomal protein L31 -
AM451_RS18440 (AM451_18445) 3784384..3784524 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
AM451_RS18445 (AM451_18450) 3784521..3785231 - 711 WP_047051118.1 GNAT family protein -
AM451_RS18450 (AM451_18455) 3785333..3786793 + 1461 WP_047051117.1 PLP-dependent aminotransferase family protein -
AM451_RS18455 (AM451_18460) 3786765..3787232 - 468 WP_017383209.1 YlaC family protein -
AM451_RS18460 (AM451_18465) 3787349..3787900 - 552 WP_017694396.1 maltose O-acetyltransferase -
AM451_RS18465 (AM451_18470) 3788109..3788327 - 219 WP_015571250.1 HHA domain-containing protein Toxin
AM451_RS18470 (AM451_18475) 3788355..3788729 - 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
AM451_RS18475 (AM451_18480) 3789239..3792385 - 3147 WP_047051116.1 multidrug efflux RND transporter permease subunit AcrB -
AM451_RS18480 (AM451_18485) 3792408..3793601 - 1194 WP_047051115.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T96470 WP_015571250.1 NZ_CP026850:c3788327-3788109 [Enterobacter cloacae complex sp.]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T96470 NZ_CP026850:c3788327-3788109 [Enterobacter cloacae complex sp.]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTCGAACGTGT
AATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTGGCTGAAC
TGACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTTTGGAAATTTGTTCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT96470 WP_006809850.1 NZ_CP026850:c3788729-3788355 [Enterobacter cloacae complex sp.]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT96470 NZ_CP026850:c3788729-3788355 [Enterobacter cloacae complex sp.]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTCAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References