96468

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/COG3657-dnstrm_HI1420
Location 3012660..3013256 Replicon chromosome
Accession NZ_CP026850
Organism Enterobacter cloacae complex sp. strain AR_0072

Toxin (Protein)


Gene name PumA Uniprot ID A0A837F9W8
Locus tag AM451_RS14735 Protein ID WP_023315213.1
Coordinates 3012954..3013256 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A837FG03
Locus tag AM451_RS14730 Protein ID WP_022650216.1
Coordinates 3012660..3012947 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM451_RS14725 (AM451_14730) 3011032..3012663 + 1632 WP_003860350.1 Na/Pi cotransporter family protein -
AM451_RS14730 (AM451_14735) 3012660..3012947 - 288 WP_022650216.1 putative addiction module antidote protein Antitoxin
AM451_RS14735 (AM451_14740) 3012954..3013256 - 303 WP_023315213.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AM451_RS14740 (AM451_14745) 3013454..3014326 + 873 WP_022646725.1 23S rRNA pseudouridine(2604) synthase RluF -
AM451_RS14745 (AM451_14750) 3014327..3014599 - 273 WP_047052050.1 DUF3811 domain-containing protein -
AM451_RS14750 (AM451_14755) 3014650..3015594 - 945 WP_032627014.1 ketopantoate/pantoate/pantothenate transporter PanS -
AM451_RS14755 (AM451_14760) 3015688..3017037 - 1350 WP_045344657.1 lysine-sensitive aspartokinase 3 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(3-93)

Antitoxin

Uncharacterized protein HI1420

(3-90)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11427.21 Da        Isoelectric Point: 10.1042

>T96468 WP_023315213.1 NZ_CP026850:c3013256-3012954 [Enterobacter cloacae complex sp.]
MKEIVQTESFRRWEQNLKDRRAKTIIASRLFRLANGLAGDIRPVGEGISELRIHFGPGYRVYFKDQGNCIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Download         Length: 303 bp

>T96468 NZ_CP026850:c3013256-3012954 [Enterobacter cloacae complex sp.]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAACTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10087.57 Da        Isoelectric Point: 5.7807

>AT96468 WP_022650216.1 NZ_CP026850:c3012947-3012660 [Enterobacter cloacae complex sp.]
MHKLTPYDPANALVDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISQQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKPSGD

Download         Length: 288 bp

>AT96468 NZ_CP026850:c3012947-3012660 [Enterobacter cloacae complex sp.]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAAACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837F9W8

Antitoxin

Source ID Structure
AlphaFold DB A0A837FG03

References