Detailed information of TA system TA096465

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 2752965..2753621
• Replicon: chromosome
• Accession: NZ_CP026850
• Organism: Enterobacter cloacae complex sp. strain AR_0072

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AM451_RS13460
• Protein ID: WP_080344062.1
• Coordinates: 2753229..2753621 (+)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: AM451_RS13455
• Protein ID: WP_047050676.1
• Coordinates: 2752965..2753225 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM451_RS13430 (AM451_13435)	2748182..2749303	-	1122	WP_047050671.1	efflux RND transporter periplasmic adaptor subunit	-
AM451_RS13435 (AM451_13440)	2749451..2750020	-	570	WP_032103771.1	TetR/AcrR family transcriptional regulator	-
AM451_RS13440 (AM451_13445)	2750206..2750625	+	420	WP_047050673.1	GNAT family N-acetyltransferase	-
AM451_RS13445 (AM451_13450)	2750622..2751269	-	648	WP_017383663.1	TetR/AcrR family transcriptional regulator	-
AM451_RS13450 (AM451_13455)	2751363..2752847	+	1485	WP_224213919.1	MDR family MFS transporter	-
AM451_RS13455 (AM451_13460)	2752965..2753225	+	261	WP_047050676.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AM451_RS13460 (AM451_13465)	2753229..2753621	+	393	WP_080344062.1	type II toxin-antitoxin system VapC family toxin	Toxin
AM451_RS13465 (AM451_13470)	2753690..2754163	-	474	WP_032674176.1	hypothetical protein	-
AM451_RS13470 (AM451_13475)	2754545..2755345	+	801	WP_047050678.1	lipoprotein NlpA	-
AM451_RS13475 (AM451_13480)	2755379..2756059	-	681	WP_047050679.1	EAL domain-containing protein	-
AM451_RS13480 (AM451_13485)	2756135..2756755	-	621	WP_047050680.1	LuxR C-terminal-related transcriptional regulator	-
AM451_RS13485 (AM451_13490)	2757296..2757961	+	666	WP_072190036.1	LuxR C-terminal-related transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-118)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14585.78 Da
• Isoelectric Point: 5.8985

>T96465 WP_080344062.1 NZ_CP026850:2753229-2753621 [Enterobacter cloacae complex sp.]
MLHMLDTNIVSHLVRQHPEVVNRYSQITPEKMCISSVTEAELLYGVAKKQNNKLHETIMEFLKTITVCAWDSEAAAIYGE
LRAAMEKKGKVMGDLDQLIAAHAISRGTTIVTNDHAFGMVQDLTVEDWTQ

Nucleotide

• Length: 393 bp

>T96465 NZ_CP026850:2753229-2753621 [Enterobacter cloacae complex sp.]
ATGCTGCATATGCTGGACACTAACATCGTAAGCCATCTGGTGAGGCAGCATCCTGAAGTTGTAAACCGATATTCGCAAAT
TACGCCTGAAAAGATGTGTATTTCAAGCGTAACGGAAGCGGAATTGCTTTACGGCGTAGCCAAAAAGCAAAACAACAAGC
TGCACGAAACCATAATGGAATTTCTTAAAACCATCACTGTCTGCGCCTGGGATAGCGAAGCCGCAGCCATCTACGGTGAA
CTGCGTGCGGCAATGGAAAAGAAAGGGAAAGTGATGGGGGATCTCGATCAGTTGATTGCCGCACATGCTATCAGCCGGGG
CACGACGATTGTCACGAACGATCATGCGTTCGGGATGGTACAGGATCTTACGGTTGAGGACTGGACTCAATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 10084.38 Da
• Isoelectric Point: 9.6899

>AT96465 WP_047050676.1 NZ_CP026850:2752965-2753225 [Enterobacter cloacae complex sp.]
MERTAKLFKKGRNQAVVLPAEFAFETESVYIRRDDEGNVVLTARSEKARHRDNFLRMLKETHVPDTFLSKEERNQSYTTR
NPLEGL

Nucleotide

• Length: 261 bp

>AT96465 NZ_CP026850:2752965-2753225 [Enterobacter cloacae complex sp.]
ATGGAACGCACAGCGAAACTATTCAAAAAAGGACGAAACCAGGCCGTGGTCTTGCCTGCGGAATTTGCGTTTGAAACGGA
GAGTGTTTATATCCGCCGGGATGACGAGGGGAATGTGGTTTTGACGGCAAGGTCCGAGAAAGCACGGCACAGGGATAACT
TTTTGCGCATGTTAAAAGAAACGCATGTGCCTGATACCTTTCTAAGTAAAGAGGAGCGCAATCAGAGCTATACGACAAGA
AACCCTCTTGAAGGGCTGTAG