Detailed information of TA system TA096464

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2602684..2603300
• Replicon: chromosome
• Accession: NZ_CP026850
• Organism: Enterobacter cloacae complex sp. strain AR_0072

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AM451_RS12725
• Protein ID: WP_077266491.1
• Coordinates: 2602684..2603055 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: AM451_RS12730
• Protein ID: WP_015569912.1
• Coordinates: 2603058..2603300 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM451_RS12710 (AM451_12715)	2600184..2601086	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
AM451_RS12715 (AM451_12720)	2601083..2601718	+	636	WP_003861958.1	formate dehydrogenase cytochrome b556 subunit	-
AM451_RS12720 (AM451_12725)	2601715..2602644	+	930	WP_022652077.1	formate dehydrogenase accessory protein FdhE	-
AM451_RS12725 (AM451_12730)	2602684..2603055	-	372	WP_077266491.1	type II toxin-antitoxin system VapC family toxin	Toxin
AM451_RS12730 (AM451_12735)	2603058..2603300	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
AM451_RS12735 (AM451_12740)	2603499..2604419	+	921	WP_047051644.1	alpha/beta hydrolase	-
AM451_RS12740 (AM451_12745)	2604428..2605369	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
AM451_RS12745 (AM451_12750)	2605414..2605851	-	438	WP_015569909.1	D-aminoacyl-tRNA deacylase	-
AM451_RS12750 (AM451_12755)	2605848..2606729	-	882	WP_003861949.1	virulence factor BrkB family protein	-
AM451_RS12755 (AM451_12760)	2606723..2607322	-	600	WP_032649898.1	glucose-1-phosphatase	-
AM451_RS12760 (AM451_12765)	2607441..2608241	-	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13723.85 Da
• Isoelectric Point: 6.4882

>T96464 WP_077266491.1 NZ_CP026850:c2603055-2602684 [Enterobacter cloacae complex sp.]
MEHMAVFDTNILIDLFNNRVEAADAIEHTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T96464 NZ_CP026850:c2603055-2602684 [Enterobacter cloacae complex sp.]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTGTTGAAGCTGCGGATGCGATTGA
GCATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTAATGGTGGGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTGATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATTGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCGCAGAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTATAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT96464 WP_015569912.1 NZ_CP026850:c2603300-2603058 [Enterobacter cloacae complex sp.]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT96464 NZ_CP026850:c2603300-2603058 [Enterobacter cloacae complex sp.]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAACCT
CAAGCAATTGCGAAATCAACCACGTGCTGAACTGCTTCGGGAAGCGATAGAACTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3