Detailed information of TA system TA096462

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA(toxin)
• Location: 980416..982199
• Replicon: chromosome
• Accession: NZ_CP026850
• Organism: Enterobacter cloacae complex sp. strain AR_0072

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: AM451_RS04690
• Protein ID: WP_047053382.1
• Coordinates: 980416..981765 (+)
• Length: 450 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: AM451_RS04695
• Protein ID: WP_047053381.1
• Coordinates: 981762..982199 (+)
• Length: 146 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM451_RS04665 (AM451_04665)	976239..977174	-	936	WP_003859306.1	tRNA dihydrouridine(16) synthase DusC	-
AM451_RS04670 (AM451_04670)	977343..977738	+	396	WP_023295055.1	CidA/LrgA family protein	-
AM451_RS04675 (AM451_04675)	977735..978430	+	696	WP_015572322.1	CidB/LrgB family autolysis modulator	-
AM451_RS04680 (AM451_04680)	978559..979443	+	885	WP_047053384.1	cytidine deaminase	-
AM451_RS04685 (AM451_04685)	979571..980281	+	711	WP_003859314.1	outer membrane permeability protein SanA	-
AM451_RS04690 (AM451_04690)	980416..981765	+	1350	WP_047053382.1	HipA domain-containing protein	Toxin
AM451_RS04695 (AM451_04695)	981762..982199	+	438	WP_047053381.1	helix-turn-helix transcriptional regulator	Antitoxin
AM451_RS04700 (AM451_04700)	982238..983248	-	1011	WP_003859318.1	galactose/methyl galactoside ABC transporter permease MglC	-
AM451_RS04705 (AM451_04705)	983264..984784	-	1521	WP_003859320.1	galactose/methyl galactoside ABC transporter ATP-binding protein MglA	-
AM451_RS04710 (AM451_04710)	984864..985862	-	999	WP_017383382.1	galactose/glucose ABC transporter substrate-binding protein MglB	-
AM451_RS04715 (AM451_04715)	986158..987180	-	1023	WP_047053378.1	HTH-type transcriptional regulator GalS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-406)

Antitoxin

• Cro/C1-type helix-turn-helix domain (78-129)

Sequences

Toxin

Protein

• Length: 450 a.a.
• Molecular weight: 51132.76 Da
• Isoelectric Point: 4.6556

>T96462 WP_047053382.1 NZ_CP026850:980416-981765 [Enterobacter cloacae complex sp.]
METLTVQAYLNETWTDIALIKYPGSEKNDWNTTQLDYLTEYAINFLDYDDFHAVSVNHPVSLFFDDHGQPGWLRFIDDII
PAGASRRYWINALDISELPVGQQNFLLLKFGTMSPVGNLRIKESVPEWNKFASTKTFTVADVIDRAADFLDYAQERGAAA
GGATGAGGEAPKLLLRCSETDAIWIDTWQNEPDNRDRYYLVKYPRGNRTEVDCNILRAEFHYYHELNAMGFSTIPVETMR
LEEGSHYPSLWLPRFDIVRNEQDQLVRLGMESVYSLLQKAPGTLLDHETTLRALINKITSSHMVQQQGYHFDIPGFVTEW
VRRDLLNIIFGNSDNHGRNTAFVKAENQIMLSPVYDFAPMKADPEGIPRTLKWSLTCESGGDYNFNTIAQTLAEWIPPAT
LLDALHETAVQLIDLPERLAARGVPEQIMEMPAMGFHYIPDKLSRWGLL

Nucleotide

• Length: 1350 bp

>T96462 NZ_CP026850:980416-981765 [Enterobacter cloacae complex sp.]
ATGGAGACGTTAACCGTACAGGCATATCTGAACGAAACATGGACGGATATCGCTCTCATCAAATATCCAGGAAGTGAAAA
AAATGACTGGAATACCACGCAGTTAGACTATCTCACTGAGTATGCGATTAATTTTCTCGACTATGATGATTTTCACGCAG
TCTCAGTCAATCACCCTGTATCACTCTTTTTTGATGACCATGGCCAACCGGGCTGGCTGCGATTTATCGACGATATTATT
CCCGCCGGAGCAAGCCGCCGTTACTGGATTAATGCGCTCGACATCAGTGAATTACCCGTTGGCCAGCAAAATTTCTTATT
GCTGAAATTTGGCACAATGTCCCCTGTGGGGAATTTGCGCATAAAAGAATCCGTCCCAGAATGGAATAAATTCGCCAGCA
CTAAAACGTTTACGGTTGCGGACGTCATCGATCGTGCGGCCGATTTTCTGGATTATGCCCAGGAAAGAGGCGCGGCGGCG
GGTGGTGCAACCGGTGCTGGCGGTGAAGCGCCTAAACTTTTGCTGCGCTGTAGTGAAACTGATGCCATCTGGATCGATAC
CTGGCAAAACGAGCCGGATAACCGGGACCGCTATTATTTGGTTAAATATCCACGCGGTAACAGGACAGAAGTTGATTGTA
ATATTCTGCGAGCTGAATTCCATTATTATCATGAGCTTAACGCGATGGGTTTTTCCACGATCCCAGTGGAAACAATGCGC
CTTGAAGAAGGTAGCCATTACCCGTCTCTGTGGTTACCGCGTTTCGACATAGTGAGAAATGAGCAGGACCAACTGGTCAG
GCTGGGTATGGAATCGGTGTATTCGCTATTACAAAAGGCACCCGGCACCCTCCTTGATCATGAGACAACGTTGCGCGCGT
TAATTAATAAAATCACCTCCAGCCATATGGTCCAGCAGCAGGGCTATCATTTTGATATTCCGGGATTTGTAACAGAATGG
GTGCGTCGCGATCTGCTAAATATTATTTTTGGAAATAGTGACAACCATGGGCGAAATACCGCCTTTGTGAAGGCTGAAAA
TCAAATTATGCTCTCCCCTGTATATGACTTTGCGCCGATGAAAGCCGATCCTGAAGGGATACCAAGAACCCTGAAGTGGT
CATTAACTTGCGAATCAGGGGGGGATTATAATTTCAACACTATCGCACAAACGCTGGCGGAGTGGATCCCTCCCGCAACA
TTACTTGATGCTCTGCATGAAACAGCCGTTCAGCTTATTGATCTCCCGGAAAGGCTGGCGGCGCGAGGCGTTCCGGAACA
GATAATGGAAATGCCCGCGATGGGTTTTCACTATATTCCGGATAAACTATCCCGTTGGGGTTTATTATGA

Antitoxin

Protein

• Length: 146 a.a.
• Molecular weight: 16161.57 Da
• Isoelectric Point: 10.5154

>AT96462 WP_047053381.1 NZ_CP026850:981762-982199 [Enterobacter cloacae complex sp.]
MKTSNEAESELLQTLAQVRSVNKKRHHDEAEDPDKPSVVKRQRVSQGLTRISTLDRQAVLHAAIRDILLGKITQGEALKR
LRVEVLGLKQDEYAKLVSVSRKTLSDVENNRGNYSADIINKIFKPFGLQTGLVPVSKSLIASLFS

Nucleotide

• Length: 438 bp

>AT96462 NZ_CP026850:981762-982199 [Enterobacter cloacae complex sp.]
ATGAAAACATCTAATGAAGCTGAATCAGAGCTTTTACAGACATTAGCTCAAGTACGCAGCGTAAATAAAAAGCGACATCA
TGACGAGGCGGAAGATCCGGACAAGCCATCGGTAGTGAAGAGGCAACGCGTTTCACAAGGCTTAACGCGGATTAGTACTC
TCGATCGCCAGGCTGTACTGCATGCAGCCATACGGGACATTTTGCTGGGGAAAATTACACAGGGAGAGGCGCTGAAAAGG
CTCAGGGTCGAGGTGTTAGGGCTGAAGCAGGATGAATATGCAAAGCTGGTCAGCGTATCCCGGAAAACATTGTCGGATGT
AGAAAATAACAGAGGCAATTATTCCGCTGACATTATAAATAAAATCTTTAAACCCTTTGGGCTTCAAACCGGACTAGTGC
CCGTTTCAAAATCGCTTATCGCTTCACTTTTCTCATAA