Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2706474..2707000 Replicon chromosome
Accession NZ_CP026846
Organism Shigella boydii strain 59-2708

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag C1P57_RS13660 Protein ID WP_000323025.1
Coordinates 2706713..2707000 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag C1P57_RS13655 Protein ID WP_000534858.1
Coordinates 2706474..2706713 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P57_RS13640 (2703832) 2703832..2705058 + 1227 Protein_2645 pitrilysin family protein -
C1P57_RS25310 (2706324) 2706324..2706449 - 126 Protein_2647 protein YdfV -
C1P57_RS13655 (2706474) 2706474..2706713 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
C1P57_RS13660 (2706713) 2706713..2707000 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
C1P57_RS13665 (2707072) 2707072..2707227 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
C1P57_RS13670 (2707445) 2707445..2707696 + 252 WP_000980987.1 protein Rem -
C1P57_RS13675 (2707763) 2707763..2708041 + 279 WP_024258459.1 hypothetical protein -
C1P57_RS13680 (2708043) 2708043..2709086 + 1044 WP_134795747.1 DUF968 domain-containing protein -
C1P57_RS13685 (2709099) 2709099..2709456 + 358 Protein_2654 RusA family crossover junction endodeoxyribonuclease -
C1P57_RS13690 (2709471) 2709471..2710292 + 822 WP_078167389.1 antitermination protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - vgrG/tssI 2695181..2713730 18549
- inside IScluster/Tn - - 2682433..2712374 29941


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T96445 WP_000323025.1 NZ_CP026846:2706713-2707000 [Shigella boydii]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T96445 NZ_CP026846:2706713-2707000 [Shigella boydii]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT96445 WP_000534858.1 NZ_CP026846:2706474-2706713 [Shigella boydii]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT96445 NZ_CP026846:2706474-2706713 [Shigella boydii]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References