Detailed information of TA system TA096429

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1007745..1008295
• Replicon: chromosome
• Accession: NZ_CP026846
• Organism: Shigella boydii strain 59-2708

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: C1P57_RS04910
• Protein ID: WP_001160972.1
• Coordinates: 1007981..1008295 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: C1P57_RS04905
• Protein ID: WP_000125567.1
• Coordinates: 1007745..1007978 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P57_RS04880 (1002894)	1002894..1003181	+	288	WP_000203729.1	ferredoxin-like protein FixX	-
C1P57_RS04885 (1003240)	1003240..1004571	+	1332	WP_134795572.1	MFS transporter	-
C1P57_RS04890 (1004682)	1004682..1005212	+	531	WP_000600743.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
C1P57_RS04895 (1005205)	1005205..1006989	+	1785	WP_119180890.1	glutathione-regulated potassium-efflux system protein KefC	-
C1P57_RS04900 (1007180)	1007180..1007659	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
C1P57_RS04905 (1007745)	1007745..1007978	+	234	WP_000125567.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
C1P57_RS04910 (1007981)	1007981..1008295	+	315	WP_001160972.1	CcdB family protein	Toxin
C1P57_RS04915 (1008292)	1008292..1009140	-	849	WP_000257196.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
C1P57_RS04920 (1009147)	1009147..1009524	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
C1P57_RS04925 (1009527)	1009527..1010348	-	822	WP_001065381.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
C1P57_RS04930 (1010345)	1010345..1011334	-	990	WP_134795573.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
C1P57_RS04935 (1011334)	1011334..1012620	-	1287	WP_078166226.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11801.86 Da
• Isoelectric Point: 9.0851

>T96429 WP_001160972.1 NZ_CP026846:1007981-1008295 [Shigella boydii]
MQFTVYRSRSRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASTHRMLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T96429 NZ_CP026846:1007981-1008295 [Shigella boydii]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCACGCACCGAATGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8544.55 Da
• Isoelectric Point: 5.8147

>AT96429 WP_000125567.1 NZ_CP026846:1007745-1007978 [Shigella boydii]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSEEYRSF

Nucleotide

• Length: 234 bp

>AT96429 NZ_CP026846:1007745-1007978 [Shigella boydii]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGAGGAGTACCGGAGCTTCTAG