Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 174573..175132 | Replicon | plasmid unnamed |
| Accession | NZ_CP026841 | ||
| Organism | Shigella dysenteriae strain ATCC 9753 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | D2AJT2 |
| Locus tag | C1P67_RS26085 | Protein ID | WP_000421262.1 |
| Coordinates | 174573..174848 (-) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | Q327A8 |
| Locus tag | C1P67_RS26090 | Protein ID | WP_001178088.1 |
| Coordinates | 174848..175132 (-) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| C1P67_RS26055 | 169575..170788 | + | 1214 | WP_171764190.1 | IS3 family transposase | - |
| C1P67_RS26065 | 170852..171982 | + | 1131 | WP_134807531.1 | IS91 family transposase | - |
| C1P67_RS26070 | 172177..173268 | - | 1092 | Protein_210 | IS66 family transposase | - |
| C1P67_RS26075 | 173276..173843 | + | 568 | Protein_211 | plasmid segregation protein ParM | - |
| C1P67_RS27565 | 173846..174028 | + | 183 | WP_241547534.1 | plasmid partitioning/stability family protein | - |
| C1P67_RS26875 | 174304..174441 | + | 138 | WP_000766699.1 | hypothetical protein | - |
| C1P67_RS26085 | 174573..174848 | - | 276 | WP_000421262.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| C1P67_RS26090 | 174848..175132 | - | 285 | WP_001178088.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| C1P67_RS26105 | 175884..176093 | + | 210 | Protein_216 | peptidoglycan-binding protein | - |
| C1P67_RS27570 | 176175..176276 | - | 102 | Protein_217 | ATP-binding protein | - |
| C1P67_RS26110 | 176428..176784 | + | 357 | WP_000239757.1 | transposase | - |
| C1P67_RS26115 | 176781..177131 | + | 351 | WP_000624671.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| C1P67_RS26120 | 177162..178754 | + | 1593 | WP_001072394.1 | IS66 family transposase | - |
| C1P67_RS26880 | 178761..178892 | - | 132 | Protein_221 | ATP-binding protein | - |
| C1P67_RS26130 | 179111..179785 | + | 675 | WP_047204740.1 | IS66-like element accessory protein TnpA | - |
| C1P67_RS26135 | 179782..179946 | + | 165 | Protein_223 | IS66 family insertion sequence element accessory protein TnpB | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | spa40 / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ipaH7.8 / ospD3/senA / ospC1 / senB / ipaH1.4 / ospE2 / ospI / ipgB2 / ospD1 / ospF / ospD2 / ospD2 / ospC4 / ospB / icsP/sopA / nleE / ospC2 / ospG / ipaH9.8 / papC / icsA/virG / virA / virA | 1..215925 | 215925 | |
| - | inside | IScluster/Tn | - | ospG / ipaH9.8 | 156870..198993 | 42123 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10766.48 Da Isoelectric Point: 9.9324
>T96425 WP_000421262.1 NZ_CP026841:c174848-174573 [Shigella dysenteriae]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T96425 NZ_CP026841:c174848-174573 [Shigella dysenteriae]
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACAGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAGAACAGGTAG
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACAGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAGAACAGGTAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10497.93 Da Isoelectric Point: 6.2150
>AT96425 WP_001178088.1 NZ_CP026841:c175132-174848 [Shigella dysenteriae]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDHPLPVPR
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDHPLPVPR
Download Length: 285 bp
>AT96425 NZ_CP026841:c175132-174848 [Shigella dysenteriae]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACCATCCGTTACCGGTGCCACGCTGA
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACCATCCGTTACCGGTGCCACGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A822PVM3 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q327A8 |