Detailed information of TA system TA096401

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 4136164..4136661
• Replicon: chromosome
• Accession: NZ_CP026839
• Organism: Shigella dysenteriae strain ATCC 9752

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: L4JJG6
• Locus tag: C1P69_RS22775
• Protein ID: WP_001173343.1
• Coordinates: 4136164..4136337 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: -
• Locus tag: C1P69_RS22780
• Protein ID: WP_151351134.1
• Coordinates: 4136365..4136661 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P69_RS21965 (4131212)	4131212..4132417	-	1206	Protein_4097	IS66 family transposase	-
C1P69_RS21970 (4132474)	4132474..4133683	+	1210	Protein_4098	IS3 family transposase	-
C1P69_RS21975 (4133726)	4133726..4134466	+	741	Protein_4099	IS3-like element IS2 family transposase	-
C1P69_RS21980 (4134528)	4134528..4136045	+	1518	WP_134804159.1	lysine--tRNA ligase	-
C1P69_RS22775 (4136164)	4136164..4136337	-	174	WP_001173343.1	type V toxin-antitoxin system toxin GhoT	Toxin
C1P69_RS22780 (4136365)	4136365..4136661	-	297	WP_151351134.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	Antitoxin
C1P69_RS21990 (4136888)	4136888..4137160	-	273	WP_000405647.1	GNAT family N-acetyltransferase	-
C1P69_RS21995 (4137172)	4137172..4137402	-	231	WP_000371700.1	4Fe-4S mono-cluster protein YjdI	-
C1P69_RS22000 (4137583)	4137583..4139214	+	1632	WP_134804160.1	sensor histidine kinase	-
C1P69_RS22005 (4139211)	4139211..4139930	+	720	WP_134804161.1	two-component system response regulator DcuR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6555.06 Da
• Isoelectric Point: 10.2288

>T96401 WP_001173343.1 NZ_CP026839:c4136337-4136164 [Shigella dysenteriae]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Nucleotide

• Length: 174 bp

>T96401 NZ_CP026839:c4136337-4136164 [Shigella dysenteriae]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11433.96 Da
• Isoelectric Point: 4.1705

>AT96401 WP_151351134.1 NZ_CP026839:c4136661-4136365 [Shigella dysenteriae]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQIYK

Nucleotide

• Length: 297 bp

>AT96401 NZ_CP026839:c4136661-4136365 [Shigella dysenteriae]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGATTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A7U9QI41