Detailed information of TA system TA096398

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3702327..3702910
• Replicon: chromosome
• Accession: NZ_CP026839
• Organism: Shigella dysenteriae strain ATCC 9752

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q31XK8
• Locus tag: C1P69_RS19775
• Protein ID: WP_000254733.1
• Coordinates: 3702327..3702662 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: C1P69_RS19780
• Protein ID: WP_089644882.1
• Coordinates: 3702662..3702910 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P69_RS19760 (3698219)	3698219..3699517	-	1299	WP_000036723.1	phosphopyruvate hydratase	-
C1P69_RS19765 (3699605)	3699605..3701242	-	1638	WP_134804059.1	CTP synthase (glutamine hydrolyzing)	-
C1P69_RS19770 (3701465)	3701465..3702256	-	792	WP_001071648.1	nucleoside triphosphate pyrophosphohydrolase	-
C1P69_RS19775 (3702327)	3702327..3702662	-	336	WP_000254733.1	endoribonuclease MazF	Toxin
C1P69_RS19780 (3702662)	3702662..3702910	-	249	WP_089644882.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
C1P69_RS19785 (3702988)	3702988..3705222	-	2235	WP_000226815.1	GTP pyrophosphokinase	-
C1P69_RS19790 (3705270)	3705270..3706570	-	1301	Protein_3681	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12167.15 Da
• Isoelectric Point: 8.7218

>T96398 WP_000254733.1 NZ_CP026839:c3702662-3702327 [Shigella dysenteriae]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKR
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T96398 NZ_CP026839:c3702662-3702327 [Shigella dysenteriae]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGA
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9341.71 Da
• Isoelectric Point: 4.4536

>AT96398 WP_089644882.1 NZ_CP026839:c3702910-3702662 [Shigella dysenteriae]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKD
VW

Nucleotide

• Length: 249 bp

>AT96398 NZ_CP026839:c3702910-3702662 [Shigella dysenteriae]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAC
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: Q31XK8