Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 76747..77522 Replicon plasmid unnamed
Accession NZ_CP026838
Organism Shigella dysenteriae strain ATCC 9752

Toxin (Protein)


Gene name tacT Uniprot ID Q326V1
Locus tag C1P69_RS00515 Protein ID WP_000405248.1
Coordinates 76747..77229 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q7BEG8
Locus tag C1P69_RS00520 Protein ID WP_010921625.1
Coordinates 77220..77522 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P69_RS23260 71805..72063 + 259 Protein_90 integrase core domain-containing protein -
C1P69_RS00480 72112..72690 + 579 WP_134803098.1 hypothetical protein -
C1P69_RS00485 72799..73057 - 259 Protein_92 IS1 family transposase -
C1P69_RS00490 73041..73604 + 564 Protein_93 transposase -
C1P69_RS00495 73729..74448 + 720 WP_001121865.1 type III secretion system effector phosphothreonine lyase -
C1P69_RS00505 75775..76113 + 339 Protein_96 integrase core domain-containing protein -
C1P69_RS00510 76156..76679 - 524 Protein_97 IS3-like element ISSfl11 family transposase -
C1P69_RS00515 76747..77229 - 483 WP_000405248.1 GNAT family N-acetyltransferase Toxin
C1P69_RS00520 77220..77522 - 303 WP_010921625.1 DUF1778 domain-containing protein Antitoxin
C1P69_RS00530 77794..78066 + 273 WP_134803100.1 hypothetical protein -
C1P69_RS00535 78047..78349 + 303 Protein_101 transposase zinc-binding domain-containing protein -
C1P69_RS00540 78388..79601 - 1214 Protein_102 IS3 family transposase -
C1P69_RS00545 79630..80565 + 936 Protein_103 IS91 family transposase -
C1P69_RS22705 80731..80865 + 135 Protein_104 IS5 family transposase -
C1P69_RS00560 81014..82468 - 1455 WP_000701110.1 type 3 secretion system effector OspC2 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospD3/senA / ospD3/senA / ospC1 / senB / ospE2 / ipaH1.4 / ipaH7.8 / ipaH4.5 / ipgB2 / ospD1 / ospF / ospC2 1..103367 103367
- inside IScluster/Tn - ipgB2 / ospD1 / ospF / ospC2 59181..83779 24598


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(29-149)

Antitoxin

Vibrio phage ICP1, Orf50

(19-94)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17736.50 Da        Isoelectric Point: 7.0382

>T96389 WP_000405248.1 NZ_CP026838:c77229-76747 [Shigella dysenteriae]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Download         Length: 483 bp

>T96389 NZ_CP026838:c77229-76747 [Shigella dysenteriae]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTGTTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTTGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11689.28 Da        Isoelectric Point: 4.9167

>AT96389 WP_010921625.1 NZ_CP026838:c77522-77220 [Shigella dysenteriae]
MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Download         Length: 303 bp

>AT96389 NZ_CP026838:c77522-77220 [Shigella dysenteriae]
ATGGAGGTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090NWA7

Antitoxin

Source ID Structure
AlphaFold DB Q7BEG8

References