Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 179604..180379 Replicon plasmid unnamed
Accession NZ_CP026837
Organism Shigella boydii strain ATCC 49812

Toxin (Protein)


Gene name tacT Uniprot ID Q326V1
Locus tag C1P56_RS27160 Protein ID WP_000405248.1
Coordinates 179604..180086 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q7BEG8
Locus tag C1P56_RS27165 Protein ID WP_010921625.1
Coordinates 180077..180379 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P56_RS27580 176027..176164 + 138 WP_011378983.1 hypothetical protein -
C1P56_RS27135 176446..176598 - 153 Protein_228 IS3 family transposase -
C1P56_RS27140 176654..177310 + 657 WP_119172631.1 type III secretion system effector cysteine methyltransferase OspZ -
C1P56_RS27150 178632..178970 + 339 Protein_231 integrase core domain-containing protein -
C1P56_RS27155 179013..179536 - 524 Protein_232 IS3-like element ISSfl11 family transposase -
C1P56_RS27160 179604..180086 - 483 WP_000405248.1 GNAT family N-acetyltransferase Toxin
C1P56_RS27165 180077..180379 - 303 WP_010921625.1 DUF1778 domain-containing protein Antitoxin
C1P56_RS27175 180651..180923 + 273 WP_000019158.1 hypothetical protein -
C1P56_RS27180 180904..182112 + 1209 WP_134796758.1 IS91 family transposase -
C1P56_RS27185 182276..182411 + 136 Protein_237 IS5 family transposase -
C1P56_RS27195 182560..184014 - 1455 WP_000701110.1 type 3 secretion system effector OspC2 -
C1P56_RS27200 184439..185283 + 845 WP_094096542.1 IS5-like element ISSfl7 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - ospG / ipaH9.8 / ospI / papC / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ospD3/senA / ospC1 / faeI / faeI / faeH / faeF / faeE / faeD / faeC / ipgB2 / ospD1 / ospF / ospD2 / ospC2 / ospC4 / ospC3 / ospB / icsP/sopA / nleE / ospC2 1..213410 213410
- inside IScluster/Tn - ipgB2 / ospD1 / ospF / ospD2 / ospC2 / ospC4 / ospC3 / ospB / icsP/sopA / nleE / ospC2 147835..194720 46885


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(29-149)

Antitoxin

Vibrio phage ICP1, Orf50

(19-94)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17736.50 Da        Isoelectric Point: 7.0382

>T96388 WP_000405248.1 NZ_CP026837:c180086-179604 [Shigella boydii]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Download         Length: 483 bp

>T96388 NZ_CP026837:c180086-179604 [Shigella boydii]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTGTTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTTGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11689.28 Da        Isoelectric Point: 4.9167

>AT96388 WP_010921625.1 NZ_CP026837:c180379-180077 [Shigella boydii]
MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Download         Length: 303 bp

>AT96388 NZ_CP026837:c180379-180077 [Shigella boydii]
ATGGAGGTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090NWA7

Antitoxin

Source ID Structure
AlphaFold DB Q7BEG8

References