Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 109562..110319 Replicon plasmid unnamed
Accession NZ_CP026837
Organism Shigella boydii strain ATCC 49812

Toxin (Protein)


Gene name tacT Uniprot ID Q31SN7
Locus tag C1P56_RS26645 Protein ID WP_000501969.1
Coordinates 109834..110319 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q31SN8
Locus tag C1P56_RS26640 Protein ID WP_011114751.1
Coordinates 109562..109846 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P56_RS26605 105598..106272 + 675 WP_119172049.1 IS66-like element accessory protein TnpA -
C1P56_RS26610 106269..106616 + 348 WP_000631709.1 IS66 family insertion sequence element accessory protein TnpB -
C1P56_RS28825 106783..107682 + 900 WP_284542724.1 IS66 family transposase -
C1P56_RS28830 107622..108116 + 495 Protein_136 IS66 family transposase -
C1P56_RS28835 108138..108242 + 105 WP_075288148.1 transposase domain-containing protein -
C1P56_RS27990 108496..108660 - 165 WP_001346193.1 hypothetical protein -
C1P56_RS26640 109562..109846 + 285 WP_011114751.1 DUF1778 domain-containing protein Antitoxin
C1P56_RS26645 109834..110319 + 486 WP_000501969.1 GNAT family N-acetyltransferase Toxin
C1P56_RS26650 110492..110824 - 333 Protein_141 IS4/IS5 family transposase -
C1P56_RS26655 110887..112043 + 1157 WP_134796752.1 IS3-like element IS600 family transposase -
C1P56_RS26660 112083..112697 - 615 Protein_143 IS4-like element ISSfl1 family transposase -
C1P56_RS26665 112706..113742 - 1037 Protein_144 IS3-like element IS911 family transposase -
C1P56_RS26675 115001..115141 - 141 Protein_146 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - ospG / ipaH9.8 / ospI / papC / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ospD3/senA / ospC1 / faeI / faeI / faeH / faeF / faeE / faeD / faeC / ipgB2 / ospD1 / ospF / ospD2 / ospC2 / ospC4 / ospC3 / ospB / icsP/sopA / nleE / ospC2 1..213410 213410
- inside IScluster/Tn - ospC3 / ospD3/senA / ospC1 100922..123408 22486


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(30-151)

Antitoxin

Vibrio phage ICP1, Orf50

(12-88)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17712.54 Da        Isoelectric Point: 10.1083

>T96387 WP_000501969.1 NZ_CP026837:109834-110319 [Shigella boydii]
MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q

Download         Length: 486 bp

>T96387 NZ_CP026837:109834-110319 [Shigella boydii]
ATGGGATGTGTAACTGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCGGTGAAGCTGTACTTGA
TGACTGGTTAAAGCAGAAGGGGCTAAAAAATCAGGCAATCGGAGCCACCCGGACTTTTGTTGTATGCAGAAAAGGTACGC
AACAAATAGTCGGTTTTTACTCACTGGCCACTGGTAGTGTCAATCATACAGAAGCTACAGGTAACCTTCGGCGCAATATG
CCAGACCCCATTCCTGTAATTATACTTGCACGCCTGGCTGTAGATGTGTCATTTCGAGGAAAAGGGCTTGGCGCGGATTT
ACTTCATGATGCAGTTCGTCGTTGTTACAGGGTTGCCGAAAATATCGGTGTGCGTGCAATTATGGTTCATGCGCTTACGG
AAAACGCAAAACAGTTTTATATTCATCATGGTTTTAAGCCATCAAAAACTCAGGTGCAAACATTGTTTCTTAAACTCCCT
CAATAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10871.52 Da        Isoelectric Point: 4.6812

>AT96387 WP_011114751.1 NZ_CP026837:109562-109846 [Shigella boydii]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Download         Length: 285 bp

>AT96387 NZ_CP026837:109562-109846 [Shigella boydii]
ATGATATACGGAGGCTTTATGAAATCAGGTGTTCAGCTCAATCTCAGAGCAAGAGAATCACAGCGGATACTCATTGATGC
TGCTGCAGAAATTCTTCATAAGTCGCGTACAGATTTCATTCTGGAAATGGCCTGTAAAGCTGCTGAAGATGTTATTCTCG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATTGAAATGCTTGATGCTCCGGTTGCAGATGATCCT
GCCATCGAAAAATTGCTGGCAAGGAAACCTCAATGGGATGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q31SN7

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CUJ1

References