Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 24510..25069 | Replicon | plasmid unnamed |
| Accession | NZ_CP026837 | ||
| Organism | Shigella boydii strain ATCC 49812 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | D2AJT2 |
| Locus tag | C1P56_RS26090 | Protein ID | WP_000421262.1 |
| Coordinates | 24510..24785 (-) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | Q327A8 |
| Locus tag | C1P56_RS26095 | Protein ID | WP_001178088.1 |
| Coordinates | 24785..25069 (-) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| C1P56_RS26055 | 19766..19873 | - | 108 | WP_023592908.1 | transposase domain-containing protein | - |
| C1P56_RS26060 | 19989..21020 | + | 1032 | WP_001121440.1 | IS630-like element IS630 family transposase | - |
| C1P56_RS26065 | 21052..21357 | - | 306 | WP_001159860.1 | type II toxin-antitoxin system toxin CcdB | - |
| C1P56_RS26070 | 21359..21577 | - | 219 | WP_000813626.1 | type II toxin-antitoxin system antitoxin CcdA | - |
| C1P56_RS26075 | 22113..23204 | - | 1092 | Protein_27 | IS66 family transposase | - |
| C1P56_RS26080 | 23212..23781 | + | 570 | Protein_28 | plasmid segregation protein ParM | - |
| C1P56_RS26085 | 23784..24134 | + | 351 | WP_134796741.1 | plasmid partitioning/stability family protein | - |
| C1P56_RS27965 | 24241..24378 | + | 138 | WP_000766699.1 | hypothetical protein | - |
| C1P56_RS26090 | 24510..24785 | - | 276 | WP_000421262.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| C1P56_RS26095 | 24785..25069 | - | 285 | WP_001178088.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| C1P56_RS26105 | 25521..25772 | + | 252 | WP_000433928.1 | transporter | - |
| C1P56_RS26110 | 25822..26031 | + | 210 | Protein_34 | peptidoglycan-binding protein | - |
| C1P56_RS26115 | 26131..26316 | - | 186 | Protein_35 | ATP-binding protein | - |
| C1P56_RS28780 | 26639..26743 | - | 105 | WP_075288148.1 | transposase domain-containing protein | - |
| C1P56_RS28785 | 26765..27259 | - | 495 | Protein_37 | IS66 family transposase | - |
| C1P56_RS28790 | 27199..28098 | - | 900 | WP_284542780.1 | IS66 family transposase | - |
| C1P56_RS26125 | 28265..28612 | - | 348 | WP_000631709.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| C1P56_RS26130 | 28609..29283 | - | 675 | WP_001088287.1 | IS66-like element accessory protein TnpA | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | ospG / ipaH9.8 / ospI / papC / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ospD3/senA / ospC1 / faeI / faeI / faeH / faeF / faeE / faeD / faeC / ipgB2 / ospD1 / ospF / ospD2 / ospC2 / ospC4 / ospC3 / ospB / icsP/sopA / nleE / ospC2 | 1..213410 | 213410 | |
| - | inside | IScluster/Tn | - | ospG / ipaH9.8 / ospI | 14515..35518 | 21003 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10766.48 Da Isoelectric Point: 9.9324
>T96386 WP_000421262.1 NZ_CP026837:c24785-24510 [Shigella boydii]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T96386 NZ_CP026837:c24785-24510 [Shigella boydii]
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAGAACAGGTAG
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAGAACAGGTAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10497.93 Da Isoelectric Point: 6.2150
>AT96386 WP_001178088.1 NZ_CP026837:c25069-24785 [Shigella boydii]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDHPLPVPR
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDHPLPVPR
Download Length: 285 bp
>AT96386 NZ_CP026837:c25069-24785 [Shigella boydii]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACCATCCGTTACCGGTGCCACGCTGA
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACCATCCGTTACCGGTGCCACGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A822PVM3 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q327A8 |