Detailed information of TA system TA096367

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1002213..1002851
• Replicon: chromosome
• Accession: NZ_CP026836
• Organism: Shigella boydii strain ATCC 49812

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: C1P56_RS05190
• Protein ID: WP_000813794.1
• Coordinates: 1002675..1002851 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C1P56_RS05185
• Protein ID: WP_001270286.1
• Coordinates: 1002213..1002629 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P56_RS05165 (997465)	997465..998410	-	946	Protein_1019	ABC transporter permease	-
C1P56_RS05170 (998411)	998411..999375	-	965	Protein_1020	ABC transporter ATP-binding protein	-
C1P56_RS05175 (999393)	999393..1000374	-	982	Protein_1021	ABC transporter substrate-binding protein	-
C1P56_RS05180 (1000792)	1000792..1002134	-	1343	Protein_1022	PLP-dependent aminotransferase family protein	-
C1P56_RS05185 (1002213)	1002213..1002629	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
C1P56_RS05190 (1002675)	1002675..1002851	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
C1P56_RS05195 (1003073)	1003073..1003303	+	231	WP_000494244.1	YncJ family protein	-
C1P56_RS05200 (1003395)	1003395..1005356	-	1962	WP_119172305.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
C1P56_RS05205 (1005429)	1005429..1005965	-	537	WP_000429147.1	DNA-binding transcriptional regulator SutR	-
C1P56_RS05210 (1006057)	1006057..1007229	+	1173	WP_235869953.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T96367 WP_000813794.1 NZ_CP026836:c1002851-1002675 [Shigella boydii]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T96367 NZ_CP026836:c1002851-1002675 [Shigella boydii]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT96367 WP_001270286.1 NZ_CP026836:c1002629-1002213 [Shigella boydii]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT96367 NZ_CP026836:c1002629-1002213 [Shigella boydii]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGAGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCACTTTATAGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB