Detailed information of TA system TA096362

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 393589..394178
• Replicon: chromosome
• Accession: NZ_CP026836
• Organism: Shigella boydii strain ATCC 49812

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A398TMA1
• Locus tag: C1P56_RS02080
• Protein ID: WP_000513533.1
• Coordinates: 393930..394178 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C1P56_RS02075
• Protein ID: WP_001016018.1
• Coordinates: 393589..393933 (-)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P56_RS02050 (389717)	389717..391223	-	1507	Protein_398	DEAD/DEAH box helicase	-
C1P56_RS28120 (391356)	391356..391592	-	237	WP_241560003.1	hypothetical protein	-
C1P56_RS28125 (391814)	391814..392071	-	258	WP_032333659.1	hypothetical protein	-
C1P56_RS02065 (392118)	392118..392357	-	240	WP_000171144.1	helix-turn-helix transcriptional regulator	-
C1P56_RS02070 (392463)	392463..393182	+	720	WP_000162429.1	LexA family transcriptional regulator	-
C1P56_RS27640 (393304)	393304..393474	+	171	WP_164834428.1	hypothetical protein	-
C1P56_RS02075 (393589)	393589..393933	-	345	WP_001016018.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C1P56_RS02080 (393930)	393930..394178	-	249	WP_000513533.1	type II toxin-antitoxin system HicA family toxin	Toxin
C1P56_RS02085 (394834)	394834..395725	+	892	Protein_406	Rha family transcriptional regulator	-
C1P56_RS02090 (395796)	395796..396008	+	213	WP_000917251.1	hypothetical protein	-
C1P56_RS02095 (396020)	396020..396301	+	282	WP_000934194.1	hypothetical protein	-
C1P56_RS02100 (396322)	396322..396603	+	282	Protein_409	host nuclease inhibitor GamL	-
C1P56_RS02105 (396621)	396621..397220	+	600	Protein_410	recombinase RecT	-
C1P56_RS02110 (397285)	397285..398441	+	1157	WP_134796284.1	IS3-like element IS600 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	384250..407941	23691

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9436.07 Da
• Isoelectric Point: 10.3195

>T96362 WP_000513533.1 NZ_CP026836:c394178-393930 [Shigella boydii]
MGKADKLLEKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL

Nucleotide

• Length: 249 bp

>T96362 NZ_CP026836:c394178-393930 [Shigella boydii]
ATGGGGAAAGCAGATAAGCTACTGGAAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAGTTGGTCGTTTTGTT
TTCCTCTCTGGGATATGTCAAAAAGGAAATGCAGGGCTCAAGAGTGCGGTTTTTCAATGCTGAAATCAACCACACCATAT
TAATGCATCGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAGCGATTAAACAGAATCTGAAAGAGGCTGGG
GTATTATGA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 13051.73 Da
• Isoelectric Point: 4.5590

>AT96362 WP_001016018.1 NZ_CP026836:c393933-393589 [Shigella boydii]
MNHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEASTLAELEQEFKTSVELYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVNHHTGA

Nucleotide

• Length: 345 bp

>AT96362 NZ_CP026836:c393933-393589 [Shigella boydii]
ATGAATCATCTAAAATATAAAGGATATTTAGGTACAGTAGAACCAGATTTTGAAAATAATATCCTGTACGGAAAACTGGC
GTTTATTCGAGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAGACATCGGTTGAGCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAGCCTGATACCCCGTTTAAAGGTGTGTTTAACGTCAGACTTGATCCAGAA
CTGCATCGTCGGGTCGCTGAAATGGCGATGGAAGAAGATTTATCACTGAACGCCTTTGTTAATAAGGCGCTGGAAAAAGA
AGTCAACCATCACACAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A398TMA1