Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 96674..97233 Replicon plasmid unnamed
Accession NZ_CP026835
Organism Shigella dysenteriae strain ATCC 49347

Toxin (Protein)


Gene name relE Uniprot ID D2AJT2
Locus tag C5B83_RS25260 Protein ID WP_000421262.1
Coordinates 96674..96949 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID Q327A8
Locus tag C5B83_RS25265 Protein ID WP_001178088.1
Coordinates 96949..97233 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5B83_RS25230 91676..92889 + 1214 WP_167544920.1 IS3 family transposase -
C5B83_RS25240 92953..94083 + 1131 WP_050546159.1 IS91 family transposase -
C5B83_RS25245 94278..95369 - 1092 Protein_122 IS66 family transposase -
C5B83_RS25250 95377..95944 + 568 Protein_123 plasmid segregation protein ParM -
C5B83_RS26330 95947..96129 + 183 WP_241547534.1 plasmid partitioning/stability family protein -
C5B83_RS25755 96405..96542 + 138 WP_000766699.1 hypothetical protein -
C5B83_RS25260 96674..96949 - 276 WP_000421262.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C5B83_RS25265 96949..97233 - 285 WP_001178088.1 ribbon-helix-helix domain-containing protein Antitoxin
C5B83_RS25275 97685..97936 + 252 WP_000433928.1 transporter -
C5B83_RS25280 97986..98195 + 210 Protein_129 peptidoglycan-binding protein -
C5B83_RS26335 98277..98378 - 102 Protein_130 ATP-binding protein -
C5B83_RS25285 98530..98886 + 357 WP_000239757.1 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospI / ipgB2 / ospD1 / ospF / ospD2 / ospB / icsP/sopA / nleE / ospC2 / ospG / ipaH9.8 1..99066 99066
- inside IScluster/Tn - ospG / ipaH9.8 85303..98886 13583


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10766.48 Da        Isoelectric Point: 9.9324

>T96360 WP_000421262.1 NZ_CP026835:c96949-96674 [Shigella dysenteriae]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T96360 NZ_CP026835:c96949-96674 [Shigella dysenteriae]
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACAGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAGAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10497.93 Da        Isoelectric Point: 6.2150

>AT96360 WP_001178088.1 NZ_CP026835:c97233-96949 [Shigella dysenteriae]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDHPLPVPR

Download         Length: 285 bp

>AT96360 NZ_CP026835:c97233-96949 [Shigella dysenteriae]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACCATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PVM3

Antitoxin

Source ID Structure
AlphaFold DB Q327A8

References