Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 54461..55236 Replicon plasmid unnamed
Accession NZ_CP026835
Organism Shigella dysenteriae strain ATCC 49347

Toxin (Protein)


Gene name tacT Uniprot ID Q326V1
Locus tag C5B83_RS24975 Protein ID WP_000405248.1
Coordinates 54461..54943 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q7BEG8
Locus tag C5B83_RS24980 Protein ID WP_010921625.1
Coordinates 54934..55236 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5B83_RS25455 50905..51042 + 138 WP_005026553.1 hypothetical protein -
C5B83_RS24950 51324..51476 - 153 Protein_70 IS3 family transposase -
C5B83_RS24955 51532..52224 + 693 WP_000625268.1 type III secretion system effector cysteine methyltransferase OspZ -
C5B83_RS24960 52248..53415 - 1168 Protein_72 IS3 family transposase -
C5B83_RS24965 53489..53827 + 339 Protein_73 integrase core domain-containing protein -
C5B83_RS24970 53870..54393 - 524 Protein_74 transposase -
C5B83_RS24975 54461..54943 - 483 WP_000405248.1 GNAT family N-acetyltransferase Toxin
C5B83_RS24980 54934..55236 - 303 WP_010921625.1 DUF1778 domain-containing protein Antitoxin
C5B83_RS24990 55508..55780 + 273 WP_000019158.1 hypothetical protein -
C5B83_RS24995 55761..56969 + 1209 WP_046891828.1 IS91-like element ISSbo1 family transposase -
C5B83_RS25340 57141..57269 + 129 Protein_79 IS5/IS1182 family transposase -
C5B83_RS25010 57418..58872 - 1455 WP_000701106.1 type 3 secretion system effector OspC2 -
C5B83_RS25015 59297..60141 + 845 WP_094096542.1 IS5-like element ISSfl7 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospI / ipgB2 / ospD1 / ospF / ospD2 / ospB / icsP/sopA / nleE / ospC2 / ospG / ipaH9.8 1..99066 99066
- inside IScluster/Tn - ipgB2 / ospD1 / ospF / ospD2 / ospB / icsP/sopA / nleE / ospC2 20419..63918 43499


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(29-149)

Antitoxin

Vibrio phage ICP1, Orf50

(19-94)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17736.50 Da        Isoelectric Point: 7.0382

>T96357 WP_000405248.1 NZ_CP026835:c54943-54461 [Shigella dysenteriae]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Download         Length: 483 bp

>T96357 NZ_CP026835:c54943-54461 [Shigella dysenteriae]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTGTTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTTGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11689.28 Da        Isoelectric Point: 4.9167

>AT96357 WP_010921625.1 NZ_CP026835:c55236-54934 [Shigella dysenteriae]
MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Download         Length: 303 bp

>AT96357 NZ_CP026835:c55236-54934 [Shigella dysenteriae]
ATGGAGGTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090NWA7

Antitoxin

Source ID Structure
AlphaFold DB Q7BEG8

References