Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2117286..2117940 Replicon chromosome
Accession NZ_CP026834
Organism Shigella dysenteriae strain ATCC 49347

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag C5B83_RS11375 Protein ID WP_000244777.1
Coordinates 2117533..2117940 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C5B83_RS11370 Protein ID WP_000354046.1
Coordinates 2117286..2117552 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5B83_RS11345 (2112574) 2112574..2113317 + 744 WP_000951947.1 SDR family oxidoreductase -
C5B83_RS11350 (2113374) 2113374..2114807 - 1434 WP_141095349.1 6-phospho-beta-glucosidase BglA -
C5B83_RS11355 (2114852) 2114852..2115163 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
C5B83_RS11360 (2115327) 2115327..2115986 + 660 WP_000250274.1 hemolysin III family protein -
C5B83_RS11365 (2116063) 2116063..2117043 - 981 WP_000886098.1 tRNA-modifying protein YgfZ -
C5B83_RS11370 (2117286) 2117286..2117552 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C5B83_RS11375 (2117533) 2117533..2117940 + 408 WP_000244777.1 protein YgfX Toxin
C5B83_RS11380 (2117980) 2117980..2118501 - 522 WP_001055874.1 flavodoxin FldB -
C5B83_RS11385 (2118613) 2118613..2119509 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C5B83_RS11390 (2119534) 2119534..2120244 + 711 WP_000715205.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C5B83_RS11395 (2120250) 2120250..2121983 + 1734 WP_129592923.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T96349 WP_000244777.1 NZ_CP026834:2117533-2117940 [Shigella dysenteriae]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T96349 NZ_CP026834:2117533-2117940 [Shigella dysenteriae]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGATTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT96349 WP_000354046.1 NZ_CP026834:2117286-2117552 [Shigella dysenteriae]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT96349 NZ_CP026834:2117286-2117552 [Shigella dysenteriae]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References