Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 188245..189020 Replicon plasmid unnamed
Accession NZ_CP026833
Organism Shigella dysenteriae strain ATCC 49346

Toxin (Protein)


Gene name tacT Uniprot ID Q326V1
Locus tag C5B81_RS25560 Protein ID WP_000405248.1
Coordinates 188538..189020 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q7BEG8
Locus tag C5B81_RS25555 Protein ID WP_010921625.1
Coordinates 188245..188547 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5B81_RS25525 184609..186063 + 1455 WP_000701106.1 type 3 secretion system effector OspC2 -
C5B81_RS25870 186212..186340 - 129 Protein_228 IS5 family transposase -
C5B81_RS25540 186512..187720 - 1209 WP_046891828.1 IS91-like element ISSbo1 family transposase -
C5B81_RS25545 187701..187973 - 273 WP_000019158.1 hypothetical protein -
C5B81_RS25555 188245..188547 + 303 WP_010921625.1 DUF1778 domain-containing protein Antitoxin
C5B81_RS25560 188538..189020 + 483 WP_000405248.1 GNAT family N-acetyltransferase Toxin
C5B81_RS25565 189088..189611 + 524 Protein_233 transposase -
C5B81_RS25570 189654..189992 - 339 Protein_234 integrase core domain-containing protein -
C5B81_RS25575 190066..191232 + 1167 Protein_235 IS3 family transposase -
C5B81_RS25580 191256..191948 - 693 WP_000625268.1 type III secretion system effector cysteine methyltransferase OspZ -
C5B81_RS25585 192004..192156 + 153 Protein_237 IS3 family transposase -
C5B81_RS26015 192438..192575 - 138 WP_005026553.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospI / ospE2 / ipaH1.4 / senB / ospC1 / ospD3/senA / ipaH7.8 / ipaH4.5 / ipaH9.8 / ospC3 / ipaJ / ipaA / ipaA / ipaD / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / icsB / ipgD / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / spa40 / virA / virA / icsA/virG / icsA/virG / papC / ipaH9.8 / ospG / ospC2 / nleE / icsP/sopA / icsP/sopA / ospB / ospD2 / ospD2 / ospF / ospD1 / ipgB2 1..224419 224419
- inside IScluster/Tn - ospC2 / nleE / icsP/sopA / icsP/sopA / ospB / ospD2 / ospD2 / ospF / ospD1 / ipgB2 179563..222203 42640


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(29-149)

Antitoxin

Vibrio phage ICP1, Orf50

(19-94)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17736.50 Da        Isoelectric Point: 7.0382

>T96333 WP_000405248.1 NZ_CP026833:188538-189020 [Shigella dysenteriae]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Download         Length: 483 bp

>T96333 NZ_CP026833:188538-189020 [Shigella dysenteriae]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTGTTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTTGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11689.28 Da        Isoelectric Point: 4.9167

>AT96333 WP_010921625.1 NZ_CP026833:188245-188547 [Shigella dysenteriae]
MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Download         Length: 303 bp

>AT96333 NZ_CP026833:188245-188547 [Shigella dysenteriae]
ATGGAGGTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090NWA7

Antitoxin

Source ID Structure
AlphaFold DB Q7BEG8

References