Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 146246..146805 | Replicon | plasmid unnamed |
| Accession | NZ_CP026833 | ||
| Organism | Shigella dysenteriae strain ATCC 49346 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | D2AJT2 |
| Locus tag | C5B81_RS25275 | Protein ID | WP_000421262.1 |
| Coordinates | 146530..146805 (+) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | Q327A8 |
| Locus tag | C5B81_RS25270 | Protein ID | WP_001178088.1 |
| Coordinates | 146246..146530 (+) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| C5B81_RS25225 | 141432..141596 | - | 165 | Protein_169 | IS66 family insertion sequence element accessory protein TnpB | - |
| C5B81_RS25230 | 141593..142267 | - | 675 | WP_046891833.1 | IS66-like element accessory protein TnpA | - |
| C5B81_RS26390 | 142486..142617 | + | 132 | Protein_171 | ATP-binding protein | - |
| C5B81_RS25240 | 142624..144215 | - | 1592 | Protein_172 | IS66 family transposase | - |
| C5B81_RS25245 | 144246..144596 | - | 351 | WP_000624671.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| C5B81_RS25250 | 144593..144949 | - | 357 | WP_000239757.1 | transposase | - |
| C5B81_RS27055 | 145101..145202 | + | 102 | Protein_175 | ATP-binding protein | - |
| C5B81_RS25255 | 145284..145493 | - | 210 | Protein_176 | peptidoglycan-binding protein | - |
| C5B81_RS25260 | 145543..145794 | - | 252 | WP_000433928.1 | transporter | - |
| C5B81_RS25270 | 146246..146530 | + | 285 | WP_001178088.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| C5B81_RS25275 | 146530..146805 | + | 276 | WP_000421262.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| C5B81_RS26395 | 146937..147074 | - | 138 | WP_000766699.1 | hypothetical protein | - |
| C5B81_RS27060 | 147350..147532 | - | 183 | WP_241547534.1 | plasmid partitioning/stability family protein | - |
| C5B81_RS25285 | 147535..148101 | - | 567 | Protein_182 | plasmid segregation protein ParM | - |
| C5B81_RS25295 | 148109..149200 | + | 1092 | Protein_183 | IS66 family transposase | - |
| C5B81_RS25300 | 149395..150525 | - | 1131 | WP_050546159.1 | IS91 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | ospI / ospE2 / ipaH1.4 / senB / ospC1 / ospD3/senA / ipaH7.8 / ipaH4.5 / ipaH9.8 / ospC3 / ipaJ / ipaA / ipaA / ipaD / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / icsB / ipgD / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / spa40 / virA / virA / icsA/virG / icsA/virG / papC / ipaH9.8 / ospG / ospC2 / nleE / icsP/sopA / icsP/sopA / ospB / ospD2 / ospD2 / ospF / ospD1 / ipgB2 | 1..224419 | 224419 | |
| - | inside | IScluster/Tn | - | ipaH9.8 / ospG | 122001..164504 | 42503 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10766.48 Da Isoelectric Point: 9.9324
>T96330 WP_000421262.1 NZ_CP026833:146530-146805 [Shigella dysenteriae]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T96330 NZ_CP026833:146530-146805 [Shigella dysenteriae]
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACAGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAGAACAGGTAG
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACAGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAGAACAGGTAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10497.93 Da Isoelectric Point: 6.2150
>AT96330 WP_001178088.1 NZ_CP026833:146246-146530 [Shigella dysenteriae]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDHPLPVPR
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDHPLPVPR
Download Length: 285 bp
>AT96330 NZ_CP026833:146246-146530 [Shigella dysenteriae]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACCATCCGTTACCGGTGCCACGCTGA
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACCATCCGTTACCGGTGCCACGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A822PVM3 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q327A8 |