Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3929941..3930595 Replicon chromosome
Accession NZ_CP026832
Organism Shigella dysenteriae strain ATCC 49346

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag C5B81_RS20730 Protein ID WP_000244777.1
Coordinates 3930188..3930595 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C5B81_RS20725 Protein ID WP_000354046.1
Coordinates 3929941..3930207 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5B81_RS20700 (3925229) 3925229..3925972 + 744 WP_000951947.1 SDR family oxidoreductase -
C5B81_RS20705 (3926029) 3926029..3927462 - 1434 WP_004986559.1 6-phospho-beta-glucosidase BglA -
C5B81_RS20710 (3927507) 3927507..3927818 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
C5B81_RS20715 (3927982) 3927982..3928641 + 660 WP_000250274.1 hemolysin III family protein -
C5B81_RS20720 (3928718) 3928718..3929698 - 981 WP_000886098.1 tRNA-modifying protein YgfZ -
C5B81_RS20725 (3929941) 3929941..3930207 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C5B81_RS20730 (3930188) 3930188..3930595 + 408 WP_000244777.1 protein YgfX Toxin
C5B81_RS20735 (3930635) 3930635..3931156 - 522 WP_001055874.1 flavodoxin FldB -
C5B81_RS20740 (3931268) 3931268..3932164 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C5B81_RS20745 (3932189) 3932189..3932899 + 711 WP_000715205.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C5B81_RS20750 (3932905) 3932905..3934638 + 1734 WP_000813209.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T96325 WP_000244777.1 NZ_CP026832:3930188-3930595 [Shigella dysenteriae]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T96325 NZ_CP026832:3930188-3930595 [Shigella dysenteriae]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGATTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT96325 WP_000354046.1 NZ_CP026832:3929941-3930207 [Shigella dysenteriae]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT96325 NZ_CP026832:3929941-3930207 [Shigella dysenteriae]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References