Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3523594..3524177 Replicon chromosome
Accession NZ_CP026832
Organism Shigella dysenteriae strain ATCC 49346

Toxin (Protein)


Gene name mazF Uniprot ID Q31XK8
Locus tag C5B81_RS18655 Protein ID WP_000254733.1
Coordinates 3523594..3523929 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag C5B81_RS18660 Protein ID WP_000581937.1
Coordinates 3523929..3524177 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5B81_RS18635 (3519101) 3519101..3519427 + 327 Protein_3578 YgcG family protein -
C5B81_RS18640 (3519487) 3519487..3520785 - 1299 WP_000036723.1 phosphopyruvate hydratase -
C5B81_RS18645 (3520873) 3520873..3522510 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
C5B81_RS18650 (3522733) 3522733..3523523 - 791 Protein_3581 nucleoside triphosphate pyrophosphohydrolase -
C5B81_RS18655 (3523594) 3523594..3523929 - 336 WP_000254733.1 endoribonuclease MazF Toxin
C5B81_RS18660 (3523929) 3523929..3524177 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
C5B81_RS18665 (3524255) 3524255..3526489 - 2235 WP_000226815.1 GTP pyrophosphokinase -
C5B81_RS18670 (3526537) 3526537..3527837 - 1301 Protein_3585 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12167.15 Da        Isoelectric Point: 8.7218

>T96323 WP_000254733.1 NZ_CP026832:c3523929-3523594 [Shigella dysenteriae]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKR
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T96323 NZ_CP026832:c3523929-3523594 [Shigella dysenteriae]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGA
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT96323 WP_000581937.1 NZ_CP026832:c3524177-3523929 [Shigella dysenteriae]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT96323 NZ_CP026832:c3524177-3523929 [Shigella dysenteriae]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q31XK8

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References