Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1044836..1045454 Replicon chromosome
Accession NZ_CP026832
Organism Shigella dysenteriae strain ATCC 49346

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C5B81_RS05250 Protein ID WP_001291435.1
Coordinates 1045236..1045454 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q325D5
Locus tag C5B81_RS05245 Protein ID WP_000344809.1
Coordinates 1044836..1045210 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5B81_RS05235 (1039926) 1039926..1041119 + 1194 WP_024258559.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
C5B81_RS05240 (1041142) 1041142..1044290 + 3149 Protein_1003 efflux RND transporter permease AcrB -
C5B81_RS05245 (1044836) 1044836..1045210 + 375 WP_000344809.1 Hha toxicity modulator TomB Antitoxin
C5B81_RS05250 (1045236) 1045236..1045454 + 219 WP_001291435.1 HHA domain-containing protein Toxin
C5B81_RS05255 (1045626) 1045626..1046177 + 552 WP_000102546.1 maltose O-acetyltransferase -
C5B81_RS05260 (1046293) 1046293..1046763 + 471 WP_000136192.1 YlaC family protein -
C5B81_RS05265 (1047078) 1047078..1048306 + 1229 WP_171765941.1 IS3-like element IS2 family transposase -
C5B81_RS05270 (1048317) 1048317..1048682 + 366 Protein_1009 CSS-motif domain-containing protein -
C5B81_RS05275 (1048747) 1048747..1049903 + 1157 WP_094110543.1 IS3-like element IS600 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T96309 WP_001291435.1 NZ_CP026832:1045236-1045454 [Shigella dysenteriae]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T96309 NZ_CP026832:1045236-1045454 [Shigella dysenteriae]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14583.42 Da        Isoelectric Point: 4.5934

>AT96309 WP_000344809.1 NZ_CP026832:1044836-1045210 [Shigella dysenteriae]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNYGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT96309 NZ_CP026832:1044836-1045210 [Shigella dysenteriae]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATTATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB Q325D5

References